FAMetA: a mass isotopologue-based tool for the comprehensive analysis of fatty acid metabolism.

Autores de IIS La Fe

• Marta Benet Giménez

  Autor

• Óscar José Juan Vidal

  Autor

• 

  Agustín Gerardo Lahoz Rodríguez

  Autor

Participantes ajenos a IIS La Fe

• Alcoriza-Balaguer MI
• García-Cañaveras JC

Grupos

• Unidad de Biomarcadores y Medicina de Precisión (UBYMP)

Abstract

The use of stable isotope tracers and mass spectrometry (MS) is the gold standard method for the analysis of fatty acid (FA) metabolism. Yet, current state-of-the-art tools provide limited and difficult-to-interpret information about FA biosynthetic routes. Here we present FAMetA, an R package and a web-based application () that uses C-13 mass isotopologue profiles to estimate FA import, de novo lipogenesis, elongation and desaturation in a user-friendly platform. The FAMetA workflow covers the required functionalities needed for MS data analyses. To illustrate its utility, different in vitro and in vivo experimental settings are used in which FA metabolism is modified. Thanks to the comprehensive characterization of FA biosynthesis and the easy-to-interpret graphical representations compared to previous tools, FAMetA discloses unnoticed insights into how cells reprogram their FA metabolism and, when combined with FASN, SCD1 and FADS2 inhibitors, it enables the identification of new FAs by the metabolic reconstruction of their synthesis route.

© The Author(s) 2023. Published by Oxford University Press.

Keywords

• fatty acids; stable isotopes; lipid metabolism; mass spectrometry; inhibitors