Enhancing Prostate Tumor Biobanking Reliability with Improved Sampling Technique and Histological Characterization.

Autores de IIS La Fe

• José Luis Ruiz Cerdá

  Autor

• Alba Loras Monfort

  Autor

• David Ramos Soler

  Autor

Participantes ajenos a IIS La Fe

• Giner Moreno E
• Quereda-Flores F
• Hernández Chinchilla JA
• Amigo Moreno R
• Perez Ruiz MD
• Carcelén López A
• Rocher Benlloch J
• Molió Sanchís P
• Casares Calzada A
• Marqués-Torrejón MÁ
• Martínez-Cadenas C

Grupos

• Endoscopia Digestiva y Hepática

  

  Leader

  Vicente Pons Beltrán

• Unidad Mixta de Investigación en Nanomedicina y Sensores

  Leader

  Ramón Martínez Máñez

Abstract

Acquiring fresh and well-characterized tumor tissue samples is critical for conducting high-quality "omics" studies. However, it can be particularly challenging in the context of prostate cancer (PC) due to the unique nature of this organ and the high heterogeneity associated with this tumor. On the other hand, histopathologically characterizing samples before their storage without causing significant tissue alterations is also an intriguing challenge. In this context, we present a new method for acquiring, mapping, characterizing, and micro-dissecting resected prostate tissue based on anatomopathological criteria. Unlike previously published protocols, this method reduces the time required for histopathological analysis of the prostate specimen without compromising its structure, which is crucial for assessing surgical margins. Furthermore, it enables the delineation and micro-macro dissection of fresh prostate tissue samples, with a focus on histological tumor areas defined by pathological criteria such as Gleason score, precursor lesions (high-grade prostatic intraepithelial neoplasia - PIN), and inflammatory lesions (prostatitis). These samples are then stored in a Biobank for subsequent research analyses.

Keywords

• QUALITY