Plasmatic Membrane Expression of Adhesion Molecules in Human Cardiac Progenitor/Stem Cells Might Explain Their Superior Cell Engraftment after Cell Transplantation.

Fecha de publicación: 10/10/2020 Fecha Ahead of Print: 10/10/2020

Autores de IIS La Fe

Imelda Ontoria Oviedo

Autor
Akaitz Dorronsoro González

Autor
Delia Castellano Izquierdo

Autor
Pilar Sepúlveda Sanchis

Autor

Participantes ajenos a IIS La Fe

Palacios I
Panadero J
Sánchez B
García-García F
López-Cerdán A
Rodríguez-Borlado L
Bernad A

Grupos

Regeneración y Trasplante Cardíaco

Leader

Pilar Sepúlveda Sanchis

Abstract

Human bone marrow mesenchymal stem cells (BM-MSCs) and cardiac progenitor/stem cells (CPCs) have been extensively studied as a potential therapeutic treatment for myocardial infarction (MI). Previous reports suggest that lower doses of CPCs are needed to improve cardiac function relative to their bone marrow counterparts. Here, we confirmed this observations and investigated the surface protein expression profile that might explain this effect. Myocardial infarction was performed in nude rats by permanent ligation of the left coronary artery. Cardiac function and infarct size before and after cell transplantation were evaluated by echocardiography and morphometry, respectively. The CPC and BM-MSC receptome were analyzed by proteomic analysis of biotin-labeled surface proteins. Rats transplanted with CPCs showed a greater improvement in cardiac function after MI than those transplanted with BM-MSCs, and this was associated with a smaller infarct size. Analysis of the receptome of CPCs and BM-MSCs showed that gene ontology biological processes and KEGG pathways associated with adhesion mechanisms were upregulated in CPCs compared with BM-MSCs. Moreover, the membrane protein interactome in CPCs showed a strong relationship with biological processes related to cell adhesion whereas the BM-MSCs interactome was more related to immune regulation processes. We conclude that the stronger capacity of CPCs over BM-MSCs to engraft in the infarcted area is likely linked to a more pronounced cell adhesion expression program.

Datos de la publicación

ISSN/ISSNe:: 1687-966X, 1687-9678
Tipo:: Article
Páginas:: 8872009-8872009
DOI:: 10.1155/2020/8872009
Factor de Impacto:: 1,205 SCImago ℠
Cuartil:: Q2 SCImago ℠

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Métricas

Filiaciones

Ontoria-Oviedo I:: Instituto de Investigación. Regenerative Medicine and Heart Transplantation Unit, Instituto de Investigación Sanitaria La Fe, 46026 Valencia, Spain
Palacios I:: Coretherapix, SLU, Tres Cantos, 28760 Madrid, Spain
Panadero J:: IGENOMIX S.L, 46980 Paterna, Valencia, Spain
Sánchez B:: Coretherapix, SLU, Tres Cantos, 28760 Madrid, Spain
García-García F:: Bioinformatics and Biostatistics Unit, Centro de Investigación Príncipe Felipe, 46012 Valencia, Spain
López-Cerdán A:: Bioinformatics and Biostatistics Unit, Centro de Investigación Príncipe Felipe, 46012 Valencia, Spain

Biomedical Imaging Unit FISABIO-CIPF, Centro de Investigación Príncipe Felipe, 46012 Valencia, Spain
Dorronsoro A:: Instituto de Investigación. Regenerative Medicine and Heart Transplantation Unit, Instituto de Investigación Sanitaria La Fe, 46026 Valencia, Spain
Castellano D:: Instituto de Investigación. Regenerative Medicine and Heart Transplantation Unit, Instituto de Investigación Sanitaria La Fe, 46026 Valencia, Spain
Rodríguez-Borlado L:: Coretherapix, SLU, Tres Cantos, 28760 Madrid, Spain
Bernad A:: Department of Immunology and Oncology, Centro Nacional de Biotecnología (CNB-CSIC), 28048 Madrid, Spain
Sepúlveda P:: Instituto de Investigación. Regenerative Medicine and Heart Transplantation Unit, Instituto de Investigación Sanitaria La Fe, 46026 Valencia, Spain