Evaluation of cytochrome P450 activities in human hepatocytes in vitro.

Autores de IIS La Fe

• María José Gómez-Lechón Moliner

  Autor

• 

  Agustín Gerardo Lahoz Rodríguez

  Autor

• José Vicente Castell Ripoll

  Autor

• María Teresa Donato Martín

  Autor

Grupos

• Hepatología experimental y Trasplante hepático

• Unidad Mixta de Investigación en Hepatología Experimental

Abstract

Major hepatic cytochrome P450 activities (CYP1A2, CYP2A6, CYP2B6, CYP2C9, CYP2C19, CYP2D6, CYP2E1, and CYP3A4) can be simultaneously examined in human hepatocytes by incubation with a cocktail of multiple specific probes. Cocktail strategy in combination with mass spectrometry is shown to be a robust, fast, and sensitive procedure for P450 activity assessment. This procedure allows a drastic reduction of the number of cells required in the assay and sample analysis time and increases throughput and reproducibility. Major applications of the probe cocktail strategy are P450 phenotyping of hepatocytes and induction studies.