Portal de investigación
c-MYC Triggers Lipid Remodelling During Early Somatic Cell Reprogramming to Pluripotency
Fecha de publicación:
Fecha Ahead of Print:
Autores de IIS La Fe
Participantes ajenos a IIS La Fe
- Prieto, Javier
- Leon, Marian
- Sendra, Ramon
- Ponsoda, Xavier
- Izpisua Belmonte, Juan Carlos
- Torres, Josema
Grupos
Abstract
Metabolic rewiring and mitochondrial dynamics remodelling are hallmarks of cell reprogramming, but the roles of the reprogramming factors in these changes are not fully understood. Here we show that c-MYC induces biosynthesis of fatty acids and increases the rate of pentose phosphate pathway. Time-course profiling of fatty acids and complex lipids during cell reprogramming using lipidomics revealed a profound remodelling of the lipid content, as well as the saturation and length of their acyl chains, in a c-MYC-dependent manner. Pluripotent cells displayed abundant cardiolipins and scarce phosphatidylcholines, with a prevalence of monounsaturated acyl chains. Cells undergoing cell reprogramming showed an increase in mitochondrial membrane potential that paralleled that of mitochondrial-specific cardiolipins. We conclude that c-MYC controls the rewiring of somatic cell metabolism early in cell reprogramming by orchestrating cell proliferation, synthesis of macromolecular components and lipid remodelling, all necessary processes for a successful phenotypic transition to pluripotency.
Datos de la publicación
- ISSN/ISSNe:
- 2629-3269, 2629-3277
- Tipo:
- Article
- Páginas:
- 2245-2261
- PubMed:
- 34476741
- Factor de Impacto:
- 1,078 SCImago ℠
- Cuartil:
- Q2 SCImago ℠
Stem Cell Reviews and Reports SPRINGER
Citas Recibidas en Web of Science: 17
Documentos
- No hay documentos
Filiaciones
Keywords
- COA-CARBOXYLASE-ALPHA; ATP CITRATE LYASE; ACETYL-COA; STEM-CELLS; SUPRAMOLECULAR ORGANIZATION; ENERGY-METABOLISM; CANCER; CARDIOLIPIN; GLYCOLYSIS; SYNTHASE