Cadaveric Adipose-Derived Stem Cells for Regenerative Medicine and Research.

Autores de IIS La Fe

• Pilar Molina Aguilar

  Autor

• Rafael Sánchez Sánchez

  Autor

• 

  Aitana Braza Boils

  Autor

• 

  Esther Zorio Grima

  Autor

Participantes ajenos a IIS La Fe

• Milian, Lara
• Oliver-Ferrandiz, Maria
• Fernandez-Sellers, Carlos
• Monzo, Ana
• Mata, Manuel

Grupos

• Cardiopatías familiares, muerte súbita y mecanismos de enfermedad (CAFAMUSME)

Abstract

Advances in regenerative medicine have enabled the search for new solutions to current health problems in so far unexplored fields. Thus, we focused on cadaveric subcutaneous fat as a promising source of adipose-derived stem cells (ADSCs) that have potential to differentiate into different cell lines. With this aim, we isolated and characterized ADSCs from cadaveric samples with a postmortem interval ranging from 30 to 55 h and evaluated their ability to differentiate into chondrocytes or osteocytes. A commercial ADSC line was used as reference. Morphological and protein expression analyses were used to confirm the final stage of differentiation. Eight out of fourteen samples from patients were suitable to complete the whole protocol. Cadaveric ADSCs exhibited features of stem cells based upon several markers: CD29 (84.49 ± 14.07%), CD105 (94.38 ± 2.09%), and CD44 (99.77 ± 0.32%). The multiparametric assessment of differentiation confirmed the generation of stable lines of chondrocytes and osteocytes. In conclusion, we provide evidence supporting the feasibility of obtaining viable postmortem human subcutaneous fat ADSCs with potential application in tissue engineering and research fields.

Keywords

• adipose-derived stem cells; mesenchymal cell; cartilage regeneration; bone regeneration; tissue engineering; in vitro disease models