Enhanced Antibacterial Activity through Silver Nanoparticles Deposited onto Carboxylated Graphene Oxide Surface

Fecha de publicación: 07/06/2022 Fecha Ahead of Print: 07/06/2022

Autores de IIS La Fe

• 

  María Ángeles Tormo Mas

  Autor

• José Manuel Pérez Royo

  Autor

Participantes ajenos a IIS La Fe

• Barjola, Arturo
• Sahuquillo, Oscar
• Bernabe-Quispe, Patricia
• Gimenez, Enrique

Grupos

• Infección Grave

  

  Investigador Principal

  María Ángeles Tormo Mas

Abstract

The strong bactericidal action of silver nanoparticles (AgNPs) is usually limited by their degree of aggregation. Deposition of AgNPs onto a graphene oxide (GO) surface to generate GO-Ag hybrids has been shown to be an effective method of controlling these aggregation problems. In this sense, a novel carboxylated graphene oxide-silver nanoparticle (GOCOOH-Ag) material has been synthesized, and their antibacterial and biofilm formation inhibitions have been studied. AgNPs decorating the GOCOOH surface achieved an average size of 6.74 +/- 0.25 nm, which was smaller than that of AgNPs deposited onto the GO surface. In addition, better distribution of AgNPs was achieved using carboxylated material. It is important to highlight the main role of the carboxylic groups in the nucleation and growth of the AgNPs that decorate the GO-based material surface. In vitro antibacterial activity and antibiofilm-forming action were tested against Gram-positive (Staphylococcus aureus and Staphylococcus epidermidis) and Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli). Both GO-Ag and GOCOOH-Ag reduced bacterial growth, analyzed by time-kill curves. However, the minimum inhibitory concentration and the minimum bactericidal concentration of GOCOOH-Ag were lower than those of GO-Ag for all strains studied, indicating that GOCOOH-Ag has better antibacterial activity. In addition, both nanomaterials prevent biofilm formation, with a higher reduction of biofilm mass and cell viability in the presence of GOCOOH-Ag. The carboxylation functionalization in GO-based materials can be applied to improve the bactericidal and antibiofilm-forming action of the AgNPs.

Keywords

• carboxylated graphene oxide; antimicrobial; biocide; silver nanoparticles