IP3 signalling regulates exogenous RNAi in Caenorhabditis elegans

Autores de IIS La Fe

• Rafael Vázquez Manrique

  Autor

• María Dolores Sequedo Pérez

  Autor

Participantes ajenos a IIS La Fe

• Nagy AI
• Lopez M
• Christov CP
• Herzog M
• Herlihy AE
• Bodak M
• Gatsi R
• Baylis HA

Grupos

• Biomedicina Molecular, Celular y Genómica

  

  Leader

  José María Millán Salvador

• Unidad mixta de investigación en enfermedades raras (CIPF)

  

  Leader

  José María Millán Salvador

Abstract

RNA interference (RNAi) is a widespread and widely exploited phenomenon. Here, we show that changing inositol 1,4,5-trisphosphate (IP3) signalling alters RNAi sensitivity in Caenorhabditis elegans. Reducing IP3 signalling enhances sensitivity to RNAi in a broad range of genes and tissues. Conversely up-regulating IP3 signalling decreases sensitivity. Tissue-specific rescue experiments suggest IP3 functions in the intestine. We also exploit IP3 signalling mutants to further enhance the sensitivity of RNAi hypersensitive strains. These results demonstrate that conserved cell signalling pathways can modify RNAi responses, implying that RNAi responses may be influenced by an animal's physiology or environment.

Keywords

• C. elegans; calcium signalling; enhanced RNAi; inositol 1,4,5-trisphosphate; RNA interference