Synergistic antimicrobial photodynamic therapy using gated mesoporous silica nanoparticles containing curcumin and polymyxin B.

Autores de IIS La Fe

• Elena Aznar Gimeno

  Autor

• Félix Sancenón Galarza

  Autor

• Ramón Martínez Máñez

  Autor

Participantes ajenos a IIS La Fe

• Medaglia, Serena
• Otri, Ismael
• Bernardos, Andrea
• Marcos, Maria Dolores

Grupos

• Unidad Mixta de Investigación en Nanomedicina y Sensores

Abstract

Photodynamic Therapy is a therapy based on combining a non-toxic compound, known as photosensitizer (PS), and irradiation with light of the appropriate wavelength to excite the PS molecule. The photon absorption by the PS leads to reactive oxygen species generation and a subsequent oxidative burst that causes cell damage and death. In this work, we report an antimicrobial nanodevice that uses the activity of curcumin (Cur) as a PS for antimicrobial Photodynamic Therapy (aPDT), based on mesoporous silica nanoparticles in which the action of the classical antibiotic PMB is synergistically combined with the aPDT properties of curcumin to combat bacteria. The synergistic effect of the designed gated device in combination with irradiation with blue LED light (470?nm) is evaluated against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus epidermidis. The results show that the nanodevice exhibits a noteworthy antibacterial activity against these microorganisms, a much more significant effect than free Cur and PMB at equivalent concentrations. Thus, 0.1?g/mL of MSNs-Cur-PMB eliminates a bacterial concentration of about 105 CFU/mL of E. coli, while 1?g/mL of MSNs-Cur-PMB is required for P. aeruginosa and S. epidermidis. In addition, antibiofilm activity against the selected bacteria was also tested. We found that 0.1?mg/mL of MSNs-Cur-PMB inhibited 99?% biofilm formation for E. coli, and 1?mg/mL of MSNs-Cur-PMB achieved 90?% and 100?% inhibition of biofilm formation for S. epidermidis and P. aeruginosa, respectively.

Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.

Keywords

• Antimicrobial photodynamic therapy, Curcumin, Mesoporous silica nanoparticles, Polymyxin B