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  3. Assessment of gold nanoparticles on human peripheral blood cells by metabolic profiling with H-1-NMR spectroscopy, a novel translational approach on a patient-specific basis

Assessment of gold nanoparticles on human peripheral blood cells by metabolic profiling with H-1-NMR spectroscopy, a novel translational approach on a patient-specific basis

Fecha de publicación:

Autores de IIS La Fe

Participantes ajenos a IIS La Fe

  • Palomino-Schatzlein, M
  • Garcia, H
  • Gutierrez-Carcedo, P
  • Herance, JAR

Grupos

Abstract

Human peripheral blood cells are relevant ex vivo models for characterizing diseases and evaluating the pharmacological effects of therapeutic interventions, as they provide a close reflection of an individual pathophysiological state. In this work, a new approach to evaluate the impact of nanoparticles on the three main fractions of human peripheral blood cells by nuclear magnetic resonance spectroscopy is shown. Thus, a comprehensive protocol has been set-up including the separation of blood cells, their in vitro treatment with nanoparticles and the extraction and characterization of metabolites by nuclear magnetic resonance. This method was applied to assess the effect of gold nanoparticles, either coated with chitosan or supported on ceria, on peripheral blood cells from healthy individuals. A clear antioxidant effect was observed for chitosan-coated gold nanoparticles by a significant increase in reduced glutathione, that was much less pronounced for gold-cerium nanoparticles. In addition, the analysis revealed significant alterations of several other pathways, which were stronger for gold-cerium nanoparticles. These results are in accordance with the toxicological data previously reported for these materials, confirming the value of the current methodology.

Datos de la publicación

ISSN/ISSNe:
1932-6203, 1932-6203

Plos One  PUBLIC LIBRARY SCIENCE

Tipo:
Article
Páginas:
-
PubMed:
28793337
Factor de Impacto:
1,164 SCImago ℠

Citas Recibidas en Web of Science: 10

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Keywords

  • OXIDATIVE STRESS; IMMUNE-SYSTEM; BIOMEDICAL APPLICATIONS; OXIDE NANOPARTICLES; LYMPHOCYTE RATIO; DRUG DISCOVERY; CANCER-THERAPY; T-LYMPHOCYTES; NEUTROPHILS; PROTEOMICS

Campos de Estudio

Proyectos y Estudios Clínicos

UNA APROXIMACIÓN ÓMICA AL DIAGNÓSTICO DE LA TUBERCULOSIS

PCIN-2013-041 . MINISTERIO DE ECONOMIA Y COMPETITIVIDAD; FUNDACIÓN PARA LA INVESTIGACIÓN DEL HOSPITAL UNIVERSITARIO LA FE DE LA COMUNIDAD VALENCIANA . 2015

HERRAMIENTAS DE RMN PARA EL DESARROLLO DE UNA PLATAFORMA PARA LA IDENTIFICACIÓN DE DIANAS, LA EVALUACIÓN DE FÁRMACOS Y LA PERSONALIZACIÓN DE TRATAMIENTOS BASADA EN APROXIMACIONES METABOLÓMICAS

Investigador Principal: ANTONIO PINEDA LUCENA

SAF2014-53977-R . MINISTERIO DE ECONOMIA Y COMPETITIVIDAD . 2015

RECALL. DESARROLLO DE PROTOTIPOS QUÍMICOS ORIENTADOS A LA GENERACIÓN DE TERAPIAS AVANZADAS PARA EL TRATAMIENTO DE LA ENFERMEDAD DE ALZHEIMER

Investigador Principal: ANTONIO PINEDA LUCENA

RTC-2016-4803-1 . MINISTERIO DE ECONOMIA Y COMPETITIVIDAD . 2016

Identificación de biotipos moleculares de cáncer de próstata como base de una medicina de precisón (BIOChiP).

Investigador Principal: LÓPEZ

PROMETEO/2016/103 . 2016

Cita

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