Development of a reliable method based on ultra-performance liquid chromatography coupled to tandem mass spectrometry to measure thiol-associated oxidative stress in whole blood samples

Fecha de publicación: 10/05/2016

Autores de IIS La Fe

Justo Javier Escobar Cubiella

Autor
Ángel Sánchez Illana

Autor
Julia Kuligowski

Autor
María Isabel Torres Cuevas

Autor
Máximo Vento Torres

Autor
Consuelo Cháfer Pericás

Autor

Participantes ajenos a IIS La Fe

Solberg R
Garberg HT
Huun MU
Saugstad OD

Grupos

Perinatología

Leader

Máximo Vento Torres

Abstract

The aminothiols are biological compounds with numerous vital functions. One of the most relevant is their role as antioxidant systems. The reduced to oxidized ratios are extremely useful indicators of oxidative stress and cellular redox status. We have validated an ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) method to determine the levels of glutathione, cysteine, homocysteine, and their respective oxidized compounds in whole blood samples. Results showed excellent linearity for all the analytes with correlation coefficients between 0.990 and 0.997, suitable precision with intra-day coefficient of variation <= 20%, and satisfactory accuracy with recoveries between 75 and 130%. The limits of detection in whole blood samples were 1.16 nmol L-1 for glutathione, 115.8 nmol L-1 for oxidized glutathione, 9.3 nmol L-1 for homocystine, 92.6 nmol L-1 for homocysteine, 347 nmol L-1 for cystine and 0.23 nmol L-1 for cysteine. The suitability of the method was ascertained in whole blood samples (n=80) from a consolidated experimental model of hypoxia-reoxygenation in newborn piglets. (C) 2016 Elsevier B.V. All rights reserved.

Datos de la publicación

ISSN/ISSNe:: 0731-7085, 1873-264X
Tipo:: Article
Páginas:: 104-112
DOI:: 10.1016/j.jpba.2016.02.007
Factor de Impacto:: 0,920 SCImago ℠
Cuartil:: Q1 SCImago ℠

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Métricas

Filiaciones

Escobar J:: Instituto de Investigación. Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain
Sánchez-Illana Á:: Instituto de Investigación. Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain
Kuligowski J:: Instituto de Investigación. Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain
Torres-Cuevas I:: Instituto de Investigación. Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain
Solberg R:: Department of Pediatric Research, University of Oslo, Oslo University Hospital, Rikshospitalet, Norway
Garberg HT:: Department of Pediatric Research, University of Oslo, Oslo University Hospital, Rikshospitalet, Norway
Huun MU:: Department of Pediatric Research, University of Oslo, Oslo University Hospital, Rikshospitalet, Norway
Saugstad OD:: Department of Pediatric Research, University of Oslo, Oslo University Hospital, Rikshospitalet, Norway
Vento M:: Division of Neonatology, University & Polytechnic Hospital La Fe, Valencia, Spain
Cháfer-Pericás C:: Instituto de Investigación. Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain.