Self-propelled enzyme-controlled IR-mesoporous silica Janus nanomotor for smart delivery

Autores de IIS La Fe

• Alba Garcia Fernandez

  Autor

• Ramón Martínez Máñez

  Autor

Participantes ajenos a IIS La Fe

• Mayol, Beatriz
• Pradana-Lopes, Sandra
• de la Torre, Cristina
• Diez, Paula
• Villalonga, Anabel
• Anillo, Carlos
• Sanchez, Alfredo
• Vilela, Diana
• Martinez-Ruiz, Paloma
• Villalonga, Reynaldo

Grupos

• Unidad Mixta de Investigación en Nanomedicina y Sensores

  Leader

  Ramón Martínez Máñez

Abstract

Here, we report the preparation of a novel Janus nanoparticle with opposite Ir and mesoporous silica nanoparticles through a partial surface masking with toposelective modification method. This nanomaterial was employed to construct an enzyme -powered nanomachine with self -propulsion properties for on -command delivery. The cargo -loaded nanoparticle was provided with a pH -sensitive gate and unit control at the mesoporous face by first attaching boronic acid residues and further immobilization of glucose oxidase through reversible boronic acid esters with the carbohydrate residues of the glycoenzyme. Addition of glucose leads to the enzymatic production of H 2 O 2 and gluconic acid, being the first compound catalytically decomposed at the Ir nanoparticle face producing O 2 and causing the nanomachine propulsion. Gluconic acid leads to a pH reduction at the nanomachine microenvironment causing the disruption of the gating mechanism with the subsequent cargo release. This work demonstrates that enzyme -mediated self -propulsion improved release efficiency being this nanomotor successfully employed for the smart release of Doxorubicin in HeLa cancer cells.

Keywords

• Nanomotor; Janus; iridium; silica; enzyme; drug; delivery