Identification of the Most Immunoreactive Antigens of Candida auris to IgGs from Systemic Infections in Mice.

Fecha de publicación: 03/05/2024 Fecha Ahead of Print: 04/04/2024

Autores de IIS La Fe

Javier Pemán García

Autor

Participantes ajenos a IIS La Fe

Areitio M
Antoran A
Rodriguez-Erenaga O
Aparicio-Fernandez L
Martin-Souto L
Buldain I
Zaldibar B
Ruiz-Gaitan A
Rementeria A
Ramirez-Garcia A

Grupos

Infección Grave

Investigador Principal

María Ángeles Tormo Mas

Abstract

The delay in making a correct diagnosis of Candida auris causes concern in the healthcare system setting, and immunoproteomics studies are important to identify immunoreactive proteins for new diagnostic strategies. In this study, immunocompetent murine systemic infections caused by non-aggregative and aggregative phenotypes of C. auris and by Candida albicans and Candida haemulonii were carried out, and the obtained sera were used to study their immunoreactivity against C. auris proteins. The results showed higher virulence, in terms of infection signs, weight loss, and histopathological damage, of the non-aggregative isolate. Moreover, C. auris was less virulent than C. albicans but more than C. haemulonii. Regarding the immunoproteomics study, 13 spots recognized by sera from mice infected with both C. auris phenotypes and analyzed by mass spectrometry corresponded to enolase, phosphoglycerate kinase, glyceraldehyde-3-phosphate dehydrogenase, and phosphoglycerate mutase. These four proteins were also recognized by sera obtained from human patients with disseminated C. auris infection but not by sera obtained from mice infected with C. albicans or Aspergillus fumigatus. Spot identification data are available via ProteomeXchange with the identifier PXD049077. In conclusion, this study showed that the identified proteins could be potential candidates to be studied as new diagnostic or even therapeutic targets for C. auris.

Datos de la publicación

ISSN/ISSNe:: 1535-3893, 1535-3907
Tipo:: Article
Páginas:: 1634-1648
DOI:: 10.1021/acs.jproteome.3c00752
Factor de Impacto:: 1,384 SCImago ℠
Cuartil:: Q1 SCImago ℠

Documentos

No hay documentos

Métricas

Filiaciones

Areitio M:: Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), 48940 Leioa, Spain
Antoran A:: Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), 48940 Leioa, Spain
Rodriguez-Erenaga O:: Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), 48940 Leioa, Spain
Aparicio-Fernandez L:: Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), 48940 Leioa, Spain
Martin-Souto L:: Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), 48940 Leioa, Spain
Buldain I:: Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy, University of the Basque Country (UPV/EHU), 01006 Vitoria-Gasteiz, Spain
Zaldibar B:: CBET Research Group, Department of Zoology and Animal Cell Biology, Faculty of Science and Technology, Research Centre for Experimental Marine Biology and Biotechnology PIE, University of the Basque Country (UPV/EHU), 48940 Leioa, Spain
Ruiz-Gaitan A:: Microbiology Department, University and Polytechnic La Fe Hospital, 46026 Valencia, Spain
Pemán J:: Microbiology Department, University and Polytechnic La Fe Hospital, 46026 Valencia, Spain
Rementeria A:: Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), 48940 Leioa, Spain
Ramirez-Garcia A:: Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), 48940 Leioa, Spain