Novel biomarkers in amniotic fluid for early assessment of intraamniotic infection

Autores de IIS La Fe

• 

  María Consuelo Cháfer Pericás

  Autor

• Ángel Sánchez Illana

  Autor

• Justo Javier Escobar Cubiella

  Autor

• María Cernada Badía

  Autor

• Elena Cubells Garcia

  Autor

• Antonio Guillermo Núñez Ramiro

  Autor

• Máximo Vento Torres

  Autor

• Julia Kuligowski

  Autor

Participantes ajenos a IIS La Fe

• Stefanovic V
• Andersson S

Grupos

• Perinatología

Abstract

Intra-amniotic infection/inflammation (IAI) is associated with preterm birth, short and long-term adverse clinical outcomes and oxidative stress. The diagnosis of IAI is based on histological and clinical findings; however, often these results are unspecific. Therefore, efforts have been directed towards validating reliable methods for patients lacking overt clinical symptoms. In this study, amniotic fluid (AF) samples were prospectively collected from 23 women grouped into two categories (with or without IAI) following clinical, microbiological and histological criteria. AFs were analyzed using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) for the determination of the following biomarkers: oxidized and nitrated tyrosines (Tyr), 8-hydroxy-2'-deoxyguanosine (8OHdG), oxidized glutathione (GSSG) and glutathione sulfonamide (GSA). 3-NO2-Tyrosine (3NO(2)-Tyr) and GSSG concentrations in AF were not identified as significantly relevant biomarkers in the presence of IAL However, inflammatory biomarkers such as GSA (p=0.002) and 3-Chloro-Tyrosine [3Cl-Tyr (p=0.049)1, and oxidative stress biomarker 8OHdG (p=0.021) were significantly increased in AF with IAI as compared to normal controls. Biomarkers of inflammation and oxidative stress determined in AF samples could represent a new approach towards an early diagnosis of IAI and subsequent chorioamnionitis in the clinical setting. (C) 2015 Elsevier Inc. All rights reserved.

Keywords

• Biomarkers; Chorioamnionitis; Intra-amniotic inflammation/infection; Oxidative stress; Liquid chromatography tandem mass spectrometry (LC-MS/MS)