Novel biomarkers in amniotic fluid for early assessment of intraamniotic infection

Fecha de publicación: 01/12/2015

Autores de IIS La Fe

Consuelo Cháfer Pericás

Autor
Ángel Sánchez Illana

Autor
Justo Javier Escobar Cubiella

Autor
María Cernada Badía

Autor
Elena Cubells Garcia

Autor
Antonio Guillermo Núñez Ramiro

Autor
Máximo Vento Torres

Autor
Julia Kuligowski

Autor

Participantes ajenos a IIS La Fe

Stefanovic V
Andersson S

Grupos

Perinatología

Leader

Máximo Vento Torres

Abstract

Intra-amniotic infection/inflammation (IAI) is associated with preterm birth, short and long-term adverse clinical outcomes and oxidative stress. The diagnosis of IAI is based on histological and clinical findings; however, often these results are unspecific. Therefore, efforts have been directed towards validating reliable methods for patients lacking overt clinical symptoms. In this study, amniotic fluid (AF) samples were prospectively collected from 23 women grouped into two categories (with or without IAI) following clinical, microbiological and histological criteria. AFs were analyzed using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) for the determination of the following biomarkers: oxidized and nitrated tyrosines (Tyr), 8-hydroxy-2'-deoxyguanosine (8OHdG), oxidized glutathione (GSSG) and glutathione sulfonamide (GSA). 3-NO2-Tyrosine (3NO(2)-Tyr) and GSSG concentrations in AF were not identified as significantly relevant biomarkers in the presence of IAL However, inflammatory biomarkers such as GSA (p=0.002) and 3-Chloro-Tyrosine [3Cl-Tyr (p=0.049)1, and oxidative stress biomarker 8OHdG (p=0.021) were significantly increased in AF with IAI as compared to normal controls. Biomarkers of inflammation and oxidative stress determined in AF samples could represent a new approach towards an early diagnosis of IAI and subsequent chorioamnionitis in the clinical setting. (C) 2015 Elsevier Inc. All rights reserved.

Datos de la publicación

ISSN/ISSNe:: 0891-5849, 1873-4596
Tipo:: Article
Páginas:: 734-740
DOI:: 10.1016/j.freeradbiomed.2015.09.014
Factor de Impacto:: 2,518 SCImago ℠
Cuartil:: Q1 SCImago ℠

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Métricas

Filiaciones

Cháfer-Pericás C:: Instituto de Investigación. Neonatal Research Unit, Health Research Institute La Fe, Valencia, Avenida Fernando Abril Martorell 106

Spain
Stefanovic V:: Department of Obstetrics and Gynecology, Fetomaternal Medical Center, Helsinki University Central Hospital and University of Helsinki, Haartmaninkatu 2, 00029, Helsinki, Finland
Sánchez-Illana Á:: Instituto de Investigación. Neonatal Research Unit, Health Research Institute La Fe, Valencia, Avenida Fernando Abril Martorell 106

Spain
Escobar J:: Instituto de Investigación. Neonatal Research Unit, Health Research Institute La Fe, Valencia, Avenida Fernando Abril Martorell 106

Spain
Cernada M:: Instituto de Investigación. Neonatal Research Unit, Health Research Institute La Fe, Valencia, Avenida Fernando Abril Martorell 106

Spain
Cubells E:: Instituto de Investigación. Neonatal Research Unit, Health Research Institute La Fe, Valencia, Avenida Fernando Abril Martorell 106

Spain
Núñez-Ramiro A:: Instituto de Investigación. Neonatal Research Unit, Health Research Institute La Fe, Valencia, Avenida Fernando Abril Martorell 106

Spain
Andersson S:: Children's Hospital, University of Helsinki and Helsinki University Hospital, Stenbäckinkatu 11, PO Box 281, 00029, Helsinki, Finland
Vento M:: Instituto de Investigación. Neonatal Research Unit, Health Research Institute La Fe, Valencia, Avenida Fernando Abril Martorell 106

46026 Valencia, Spain.
Kuligowski J:: Instituto de Investigación. Neonatal Research Unit, Health Research Institute La Fe, Valencia, Avenida Fernando Abril Martorell 106

Spain.