Adalimumab Reduces Photoreceptor Cell Death in A Mouse Model of Retinal Degeneration

Autores de IIS La Fe

• Cristina Martinez Fernandez De La Camar

  Autor

• Lorena Olivares González

  Autor

• David Hervás Marín

  Autor

• David Salom Alonso

  Autor

• 

  José María Millán Salvador

  Autor

• Regina Rodrigo Nicolás

  Autor

Participantes ajenos a IIS La Fe

• Hernández-Pinto AM
• Cuevas-Martín C
• Sánchez-Aragó M
• Cuezva JM
• de la Rosa EJ

Grupos

• 

  Biomedicina Molecular, Celular y Genómica

Abstract

Growing evidence suggests that inflammation is involved in the progression of retinitis pigmentosa (RP) both in patients and in animal models. The aim of this study was to investigate the effect of Adalimumab, a monoclonal anti-TNF alpha antibody, on retinal degeneration in a murine model of human autosomal recessive RP, the rd10 mice at postnatal day (P) 18. In our housing conditions, rd10 retinas were seriously damaged at P18. Adalimumab reduced photoreceptor cell death, as determined by scoring the number of TUNEL-positive cells. In addition, nuclear poly (ADP) ribose (PAR) content, an indirect measure of PAR polymerase (PARP) activity, was also reduced after treatment. The blockade of TNF alpha ameliorated reactive gliosis, as visualized by decreased GFAP and IBA1 immunolabelling (Muller cell and microglial markers, respectively) and decreased up-regulation of TNF alpha gene expression. Adalimumab also improved antioxidant response by restoring total antioxidant capacity and superoxide dismutase activity. Finally, we observed that Adalimumab normalized energetic and metabolic pattern in rd10 mouse retinas. Our study suggests that the TNF alpha blockade could be a successful therapeutic approach to increase photoreceptor survival during the progression of RP. Further studies are needed to characterize its effect along the progression of the disease.

Keywords

• CHRONIC INFLAMMATORY REACTION; HUMAN RETINITIS-PIGMENTOSA; NECROSIS-FACTOR-ALPHA; TNF-ALPHA; MACULAR DEGENERATION; OXIDATIVE STRESS; RHEUMATOID-ARTHRITIS; PORCINE RETINA; BETA-SUBUNIT; ACTIVATION