Novel nonclassic progesterone receptor PGRMC1 pulldown-precipitated proteins reveal a key role during human decidualization

Autores de IIS La Fe

• Stefania Salsano

  Autor

• Roberto González Martín

  Autor

• Alicia Quiñonero Villora

  Autor

• Ana Pilar Gómez Escribano

  Autor

• Silvia Pérez Deben

  Autor

• Francisco Domínguez Hernández

  Autor

Participantes ajenos a IIS La Fe

• Lopez-Martin, S
• Yanez-Mo, M

Grupos

• Biología Reproductiva y Bioingeniería en Reproducción Humana

  

  Leader

  Nicolás Garrido Puchalt

• Biomedicina Molecular, Celular y Genómica

  

  Leader

  José María Millán Salvador

• Unidad mixta de investigación en enfermedades raras (CIPF)

  

  Leader

  José María Millán Salvador

Abstract

Objective: To investigate PGRMC1-precipitating proteins in human endometrial stromal cells (ESC) to understand its role during in vitro decidualization. Design: Prospective observational study. Setting: Academic fertility center. Patient(s): Fifteen fertile oocyte donors. Intervention(s): Isolated ESCs decidualized in vitro and used in pulldown assays. Main Outcome Measure(s): GST-PGRMC1-precipitated proteins identified in nondecidualized ESC (ndESC) and ESC decidualized via a long (8 days) or short (4 days) decidualization protocol (dESC). Result(s): Using pulldown assays and mass spectrometry, decidualization was evaluated by prolactin secretion (ELISA) and cytoskeleton morphology (F-actin staining). The protein interactions were validated by colocalization and coimmunoprecipitation. The pulldown and mass spectrometry analysis identified 21, 24, and 24 new significant GST-PGRMC1-precipitated proteins in ndESC, long dESC, and short dESC, respectively, compared with controls. The functional annotation analysis categorized these proteins mainly into endomembrane system and mitochondria cellular components, both related to adenosine triphosphate (ATP) generation and transport activity, protein biosynthesis and posttranslational processing, vesicle trafficking, and protection against oxidative stress activities. Monoamine oxidase B (MAOB) and B-cell receptor-associated protein 31 (BAP31) were identified in dESC from both decidualization protocols. PGRIVIC1-MA0B/BAP31 interactions were confirmed by immunofluorescence and coimmunoprecipitation in dESC. Conclusion(s): Novel GST-PGRMC1-precipitated proteins discovered in ESC suggest that this protein is implicated in deep remodeling of ESC during decidualization and aggregates mainly with proteins involved in biosynthesis, intracellular transport, and mitochondrial activity. (C) 2020 by American Society for Reproductive Medicine.

Keywords

• Decidualization; endometrium; PGRMC1; progesterone receptor