Portal de investigación
Bioengineering strategies of the uterus towards improving current investigative models and female reproductive health
Autores de IIS La Fe
Grupos
Abstract
Ever since the inception of artificial reproductive technologies (ART), new advances have been developed in the lab and translated to the clinic to improve the reproductive outcome of patients. Tissue engineering (TE) adopts ideas and concepts from biology, bioengineering and material science amongst others, resulting in a promising and burgeoning multidisciplinary field of investigation within regenerative medicine. The main objective of the work presented in this thesis was to use TE based approaches to create different types of natural biomaterials obtained from decellularized porcine or rabbit uteri. We investigated if these different bioscaffolds could improve current investigative in vitro models while also showing potential to be used as therapeutic solutions. Decellularized whole organs are acellular vascularized scaffolds that could be used to create tissue-engineered, transplantable organs. However, they can also be processed further into thin sections, ECM hydrogels and coatings, and were used as biocompatible tissue-specific substrates for cell and embryo culture. Two animal models were used, the pig model demonstrated that our perfusion-based protocol (with or without a freeze/thaw step) successfully decellularizes large uteri, yielding a biocompatible material. Secondly, w e adapted this protocol for the rabbit uterus and converted the acellular endometrium into tissue-specific ECM hydrogels and coatings. After characterization of these substrates their effect on in vitro embryo development was also examined. While DC organs could one day be used to resolve the main issues plaguing transplantations, endometrial ECM sections, hydrogels and coatings have shown the potential to become a platform used in the culture of stem/progenitor cells and primary culture cells to better maintain their tissue-specific phenotype, improving in vitro models. Furthermore, ECM hydrogels could possibly be used in the future in vivo, as part of a treatment of Asherman's syndrome and endometrial atrophy.
Filiaciones
Keywords
- Tissue engineering; decellularization; uterine bioengineering; ECM hydrogel; embryo culture
Proyectos asociados
ESTUDIO DE LA ENFERMEDAD METASTASICA EN EL TEJIDO OVARICO CRIOPRESERVADO DE MUJERES CON CANCER DE MAMA
Investigador Principal: ANTONIO PELLICER MARTÍNEZ
PI08/90483 . INSTITUTO DE SALUD CARLOS III . 2009
CONTRATO POSTDOCTORAL DE INVESTIGACION SARA BORRELL
CD11/00292 . INSTITUTO DE SALUD CARLOS III; FUNDACIÓN PARA LA INVESTIGACIÓN DEL HOSPITAL UNIVERSITARIO LA FE DE LA COMUNIDAD VALENCIANA . 2012
CONTRATO POSTDOCTORAL DE INVESTIGACION SARA BORELL
CD12/00568 . INSTITUTO DE SALUD CARLOS III; FUNDACIÓN PARA LA INVESTIGACIÓN DEL HOSPITAL UNIVERSITARIO LA FE DE LA COMUNIDAD VALENCIANA . 2013
CONTRATO POST FSE (RIO HORTEGA)
CM13/00191 . INSTITUTO DE SALUD CARLOS III; FUNDACIÓN PARA LA INVESTIGACIÓN DEL HOSPITAL UNIVERSITARIO LA FE DE LA COMUNIDAD VALENCIANA . 2014
CONTRATO POSTDOCTORAL DE INVESTIGACION SARA BORRELL
Investigador Principal: ANTONIO PELLICER MARTÍNEZ
CD15/00058 . INSTITUTO DE SALUD CARLOS III; FUNDACIÓN PARA LA INVESTIGACIÓN DEL HOSPITAL UNIVERSITARIO LA FE DE LA COMUNIDAD VALENCIANA . 2016
TRAMIENTO COMBINADO DE VITAMINA D CON AGNRH SOBRE EL CRECIMIENTO DE LOS MIOMAS UTERINOS
Investigador Principal: ANTONIO PELLICER MARTÍNEZ
PI15/00312 . INSTITUTO DE SALUD CARLOS III . 2016
Alternativas para la Preservacion de la fertilidad en pacientes con leucemia aguda. Maduracion in vivo mediante xenotransplante.
Investigador Principal: ANTONIO PELLICER MARTÍNEZ
GRISOLIAP/2018/029 . CONSELLERIA DE EDUCACION . 2018
Estudio de las modificaciones epigenéticas como posibles dianas terapéuticas para tratar los miomas uterinos.
Investigador Principal: ANTONIO PELLICER MARTÍNEZ
PI18/00323 . INSTITUTO DE SALUD CARLOS III . 2019
Aplicaciones de los cultivos tridimensionales en reproducción.
Investigador Principal: HORTENSIA FERRERO CHAFER
ACIF/2017/118 . GENERALITAT VALENCIANA; CONSELLERIA DE EDUCACION . 2018
Identificación de factores regenerativos en medicina reproductiva y su aplicación como futura herramienta terapéutica para la reparación del endometrio.
Investigador Principal: IRENE CERVELLÓ ALCARAZ
PI17/01039 . INSTITUTO DE SALUD CARLOS III . 2018
ESTUDIO CLÍNICO PILOTO PARA EVALUAR EL EFECTO DE LA VITAMINA D SOBRE EL TAMAÑO DE LOS MIOMAS EN MUJERES CON DEFICIENCIA DE VITAMINA D.
Investigador Principal: ANTONIO PELLICER MARTÍNEZ
IIS-COL-2018-01 . 2019
Cita
Campo H,Cervello I,Pellicer A. Bioengineering strategies of the uterus towards improving current investigative models and female reproductive health. Facts Views Vis Obgyn. 2019. 11. (1):p. 87-99.