Portal de investigación
Evaluation of the antiproliferative, proapoptotic, and antiangiogenic effects of a double-stranded RNA mimic complexed with polycations in an experimental mouse model of leiomyoma
Fecha de publicación:
Autores de IIS La Fe
Participantes ajenos a IIS La Fe
- Garcia-Pascual CM
- Ferrero H
- Martinez J
- Villanueva A
- Pozuelo-Rubio M
- Soengas M
- Tormo D
- Simon C
- Gomez R
Grupos
Abstract
Objective: To assess the antiproliferative, proapoptotic, and antiangiogenic effects of the double-stranded RNA mimic polyinosinepolycytidylic acid (pIC) complexed with polyethylenimine [pIC(PEI)] in xenografted human leiomyomas. Design: Heterologous leiomyoma mouse model. Setting: University-affiliated infertility center. Animal(s): Ovariectomized and hormone-replaced nude mice (n = 16) who received human leiomyoma fragment transplantation. Intervention(s): Leiomyoma fragments placed in the peritoneum of 5-week-old nude female mice and treated with the vehicle (n = 8) or 0.6 mg/kg [pIC(PEI)] (n = 8) for 4 weeks. Main Outcome Measure(s): The size of the leiomyoma implants, and cellular proliferation (Ki67), vascularization (PECAM), and apoptosis (OH-ends) assessed by quantitative immunohistochemical/immunofluorescent analysis of the recovered implants. Result(s): No significant differences were observed in the size of the leiomyoma implants between groups. Vascularization and proliferation were significantly decreased, and apoptosis was increased in the [pIC(PEI)]-treated group versus control. Conclusion(s): We hypothesize that the antiangiogenic and apoptotic effects exerted by [pIC(PEI)] might lead to a decrease in lesion size in this animal model if the compound is administered for longer periods of time. This study provides promising data on [pIC(PEI)] as a potential novel therapeutic agent against human leiomyoma. (C) 2016 by American Society for Reproductive Medicine.
Datos de la publicación
- ISSN/ISSNe:
- 0015-0282, 1556-5653
- Tipo:
- Article
- Páginas:
- 529-538
- Factor de Impacto:
- 2,237 SCImago ℠
- Cuartil:
- Q1 SCImago ℠
FERTILITY AND STERILITY ELSEVIER SCIENCE INC
Citas Recibidas en Web of Science: 4
Documentos
- No hay documentos
Filiaciones
Keywords
- Angiogenesis; apoptosis; leiomyoma; mouse model; [pIC(PEI)]
Proyectos asociados
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ESTUDIO ALEATORIZADO, DOBLE CIEGO, CONTROLADO CON PLACEBO, PARA EVALUAR SEGURIDAD Y EFICACIA DE ELAGOLIX EN PARTICIPANTES CON DOLOR MODERADO O SEVERO ASOCIADO A ENDOMETRIOSIS.
Investigador Principal: VICENTE PAYÁ AMATE
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EXTENSION STUDY TO EVALUATE THE LONG-TERM SAFETY AND EFFICACY OF ELAGOLIX IN SUBJECTS WITH MODERATE TO SEVERE ENDOMETRIOSIS-ASSOCIATED PAIN. / ESTUDIO DE EXTENSIÓN PARA EVALUAR LA SEGURIDAD Y LA EFICACIA A LARGO PLAZO DE ELAGOLIX EN PARTICIPANTES CON DOLO R DE MODERADO A SEVERO ASOCIADO A ENDOMETRIOSIS.
Investigador Principal: VICENTE PAYÁ AMATE
M12-821 . 2014
Cita
Garcia CM,Ferrero H,Juarez I,Martinez J,Villanueva A,Pozuelo M,Soengas M,Tormo D,Simon C,Gomez R,Pellicer A. Evaluation of the antiproliferative, proapoptotic, and antiangiogenic effects of a double-stranded RNA mimic complexed with polycations in an experimental mouse model of leiomyoma. Fertil Steril. 2016. 105. (2):p. 529-538. IF:4,373. (1).