Portal de investigación
Human Endometrial Reconstitution From Somatic Stem Cells: The Importance of Niche-Like Cells
Autores de IIS La Fe
Participantes ajenos a IIS La Fe
- Lopez-Perez, N
- Gil-Sanchis, C
- Diaz, A
Grupos
Abstract
Endometrial regeneration has long been proposed to be mediated by stem cells, but the isolation of endometrial stem cells has been hampered by a lack of validated markers. Specific markers would enable isolation of these stem cells, thereby promoting advancements in regenerative medicine for the treatment of endometrial diseases and dysfunctions. We sought to investigate the regenerative ability of human endometrial positive for sushi domain containing 2/intercellular adhesion molecule 1 (SUSD2(+)/ICAM1(+)) cells and Side Population cell lines in a xenograft mice model. The injection of total endometrial cell suspensions and Side Population cell lines under kidney capsules induced neoformation of human endometrium verified by the presence of typical endometrial markers (vimentin, cytokeratin 18, and progesterone receptor) by immunofluorescence. Total endometrial cell types promoted a better reconstitution in comparison to injecting ICAM1(+) and SUSD2(+) cells alone. The endometrial fraction is probably acting as a niche, resulting in increased reconstruction efficiency of pure fractions. Human engrafted cells were localized near blood vessels and induced the proliferation of surrounding cells. Our results suggest that human endometrial Side Population, a heterogeneous population possibly harboring endometrial stem cells, has the optimum capacity to regenerate endometrial-like tissue. In contrast, cells positive for single stem cell markers SUSD2 and ICAM1 have minimally functional regenerative capacities in the absence of niche-like cells.
Datos de la publicación
- ISSN/ISSNe:
- 1933-7191, 1933-7205
- Tipo:
- Article
- Páginas:
- 77-87
- Factor de Impacto:
- 0,905 SCImago ℠
- Cuartil:
- Q2 SCImago ℠
REPRODUCTIVE SCIENCES SAGE PUBLICATIONS INC
Citas Recibidas en Web of Science: 5
Documentos
- No hay documentos
Filiaciones
Keywords
- endometrium; stem cell markers; SUSD2/ICAM1; Side Population; niche
Proyectos asociados
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PI08/90483 . INSTITUTO DE SALUD CARLOS III . 2009
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CONTRATO POSTDOCTORAL DE INVESTIGACION SARA BORELL
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CONTRATO POST FSE (RIO HORTEGA)
CM13/00191 . INSTITUTO DE SALUD CARLOS III; FUNDACIÓN PARA LA INVESTIGACIÓN DEL HOSPITAL UNIVERSITARIO LA FE DE LA COMUNIDAD VALENCIANA . 2014
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Investigador Principal: ANTONIO PELLICER MARTÍNEZ
CD15/00058 . INSTITUTO DE SALUD CARLOS III; FUNDACIÓN PARA LA INVESTIGACIÓN DEL HOSPITAL UNIVERSITARIO LA FE DE LA COMUNIDAD VALENCIANA . 2016
TRAMIENTO COMBINADO DE VITAMINA D CON AGNRH SOBRE EL CRECIMIENTO DE LOS MIOMAS UTERINOS
Investigador Principal: ANTONIO PELLICER MARTÍNEZ
PI15/00312 . INSTITUTO DE SALUD CARLOS III . 2016
Estudio de las modificaciones epigenéticas como posibles dianas terapéuticas para tratar los miomas uterinos.
Investigador Principal: ANTONIO PELLICER MARTÍNEZ
PI18/00323 . INSTITUTO DE SALUD CARLOS III . 2019
Identificación de factores regenerativos en medicina reproductiva y su aplicación como futura herramienta terapéutica para la reparación del endometrio.
Investigador Principal: IRENE CERVELLÓ ALCARAZ
PI17/01039 . INSTITUTO DE SALUD CARLOS III . 2018
Influencia de los disruptores endocrinos sobre la receptividad endometrial humana y su relación con el fallo de impantación y la endometriosis.
Investigador Principal: FRANCISCO DOMÍNGUEZ HERNÁNDEZ
PI17/00931 . INSTITUTO DE SALUD CARLOS III . 2018
Cita
Lopez N,Gil C,Ferrero H,Faus A,Diaz A,Pellicer A,Cervello I,Simon C. Human Endometrial Reconstitution From Somatic Stem Cells: The Importance of Niche-Like Cells. Reprod Sci. 2019. 26. (1):p. 77-87. IF:2,616. (2).