Fertility rescue and ovarian follicle growth promotion by bone marrow stem cell infusion

Data de publicació: 01/05/2018

Autors de IIS La Fe

Sonia Herraiz Raya

Autor
Ana Buigues Lafuente

Autor
César Díaz García

Autor
Mónica Romeu Villarroya

Autor
Vicenta Susana Martínez Cuenca

Autor
Inés Gómez Seguí

Autor
Antonio Pellicer Martínez

Autor

Autors aliens a IIS La Fe

Simon, C
Hsueh, AJ

Grups d'Investigació

Hematología y Hemoterapia

Investigador Principal

Javier De La Rubia Comos
Medicina Reproductiva

Investigador Principal

José Morales Roselló

Abstract

Objective: To assess if infusion of human bone marrow-derived stem cells (BMDSCs) could promote follicle development in patients with impaired ovarian functions. Design: Experimental design. Setting: University research laboratories. Animal(s): Immunodeficient NOD/SCID female mice. Intervention(s): Human BMDSCs were injected into mice with chemotherapy-induced ovarian damage and into immunodeficient mice xenografted with human cortex from poor-responder patients (PRs). Main Outcome Measure(s): Follicle development, ovulation, and offspring. Apoptosis, proliferation, and vascularization were evaluated in mouse and human ovarian stroma. Result(s): Fertility rescue and spontaneous pregnancies were achieved in mice ovaries mimicking PRs and ovarian insufficiency, induced by chemotherapy, after BMDSC infusion. Furthermore, BMDSC treatment resulted in production of higher numbers of preovulatory follicles, metaphase II oocytes, 2-cell embryos, and healthy pups. Stem cells promoted ovarian vascularization and cell proliferation, along with reduced apoptosis. In xenografted human ovarian tissues from PRs, infusion of BMDSCs and their CD133+ fraction led to their engraftment close to follicles, resulting in promotion of follicular growth, increases in E-2 secretion, and enhanced local vascularization. Conclusion(s): Our results raised the possibility that promoting ovarian angiogenesis by BMDSC infusion could be an alternative approach to improve follicular development in women with impaired ovarian function. (C) 2018 by American Society for Reproductive Medicine.

Dades de la publicació

ISSN/ISSNe:: 0015-0282, 1556-5653
Tipus:: Article
Pàgines:: 908-908
DOI:: 10.1016/j.fertnstert.2018.01.004
PubMed:: 29576341
Factor d'Impacte:: 2,332 SCImago ℠
Quartil:: Q1 SCImago ℠

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Filiacions

Herraiz, S:: IVI Fdn, Ave Fernando Abril Martorell 106 TorreA Planta1, Valencia 46026, Spain

Instituto de Investigación. Reprod Med Res Grp, Valencia, Spain

Univ Valencia, Sch Med, Dept Pediat Obstet & Gynecol, Valencia, Spain
Buigues, A:: IVI Fdn, Ave Fernando Abril Martorell 106 TorreA Planta1, Valencia 46026, Spain

Univ Valencia, Sch Med, Dept Pediat Obstet & Gynecol, Valencia, Spain
Diaz-Garcia, C:: Instituto de Investigación. Reprod Med Res Grp, Valencia, Spain

Univ Valencia, Sch Med, Dept Pediat Obstet & Gynecol, Valencia, Spain

IVI London, London, England
Romeu, M:: Instituto de Investigación. Reprod Med Res Grp, Valencia, Spain
Martinez, S:: Instituto de Investigación. Reprod Med Res Grp, Valencia, Spain
Gomez-Segui, I:: La Fe Univ Hosp, Dept Hematol, Valencia, Spain
Simon, C:: Univ Valencia, Sch Med, Dept Pediat Obstet & Gynecol, Valencia, Spain

Inst Univ IVI INCLIVA, Valencia, Spain

Igenomix, Paterna, Spain

Stanford Univ, Sch Med, Dept Obstet & Gynecol, Stanford, CA 94305 USA
Hsueh, AJ:: Stanford Univ, Sch Med, Dept Obstet & Gynecol, Stanford, CA 94305 USA
Pellicer, A:: IVI Fdn, Ave Fernando Abril Martorell 106 TorreA Planta1, Valencia 46026, Spain

Instituto de Investigación. Reprod Med Res Grp, Valencia, Spain