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Contribution of molecular analysis to the typification of the non-functioning pituitary adenomas

Fecha de publicación:

Autores de IIS La Fe

Participantes ajenos a IIS La Fe

  • Sanchez-Tejada, L
  • Sanchez-Ortiga, R
  • Lamas, C
  • Riesgo, P
  • Fajardo, C
  • Aranda, FI
  • Pico, A

Grupos

Abstract

Aim The WHO Classification of Tumours of Endocrine Organs considers the inmunohistochemical characterization of pituitary adenomas (PA) as mandatory for patient diagnosis. Recent advances in the knowledge of the molecular patterns of these tumours could complement this classification with gene expression profiling. Methods Within the context of the Spanish Molecular Registry of Pituitary Adenomas (REMAH), a multicentre clinical- basic research project, we analysed the molecular phenotype of 142 PAs with complete IHC and clinical information. Gene expression levels of all pituitary hormones, type 1 corticotrophin- releasing hormone receptor, dopamine receptors and arginine vasopressin receptor 1b were measured by quantitative real- time polymerase chain reaction. In addition, we used three housekeeping genes for normalization and a pool of nine healthy pituitary glands from autopsies as calibration reference standard. Results Based on the clinically functioning PA (FPA: somatotroph, corticotroph, thyrotroph and lactotroph adenomas), we established the interquartile range of relative expression for all genes studied in each PA subtype. That allowed molecularly the different PA subtypes, including the clinically non- functioning PA (NFPA). Afterwards, we estimated the concordance of the molecular and immunohistochemical classification with clinical diagnosis in FPA and between them in NFPA. The kappa values were higher in molecular than in immunohistochemical classification in FPA and showed a bad concordance in all NFPA subtypes. Conclusions According to these results, the molecular characterization of the PA complements the IHC analysis, allowing a better typification of the NFPA.

Datos de la publicación

ISSN/ISSNe:
1932-6203, 1932-6203

Plos One  PUBLIC LIBRARY SCIENCE

Tipo:
Article
Páginas:
-
Factor de Impacto:
1,164 SCImago ℠

Citas Recibidas en Web of Science: 18

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ESTUDIO FASE III, MULTICENTRICO, ALEATORIZADO, DOBLE CIEGO DE 48 SEMANAS, CON UN PERIDO INICIAL DE 12 SEMANAS CONTROLADO CON PLACEBO, PARA EVALUAR LA SEGURIDAD Y LA EFICACIA DE OSILODROSTAT EN PACIENTES CON ENFERMEDADES DE CUSHING.

Investigador Principal: ROSA CÁMARA GÓMEZ

CLCI699C2302 . 2016

ESTUDIO OBSERVACIONAL, TRASVERSAL Y MULTICÉNTRICO PARA VALORAR EL CUMPLIMENTO TERAPÉUTICO CON SOMAVERT® EN PACIENTES CON ACROMEGALIA EN CONDICIONES DE PRÁCTICA CLÍNICA HABITUAL.

Investigador Principal: ROSA CÁMARA GÓMEZ

PFI-PEG-2014-01 . 2015

ESTUDIO OBSERVACIONAL, MULTICÉNTRICO INTERNACIONAL, PARA EVALUAR LA EFECTIVIDAD EN LA PRÁCTICA CLÍNICA COTIDIANA DE LA LANREOTIDA AUTOGEL 120 MG, EN INTERVALOS POSOLÓGICOS PROLONGADOS (>4 SEMANAS), PARA EL TRATAMIENTO DE LA ACROMEGALIA: ESTUDIO SOMACROL.

Investigador Principal: ROSA CÁMARA GÓMEZ

IPS-SOM-2015-01 . 2016

ESTUDIO MUNDIAL SOBRE EL CONTROL Y LAS COMPLICACIONES DEL HIPOTITUITARISMO (HYPOCCS).

Investigador Principal: JUAN FRANCISCO MERINO TORRES

B9R-MC-GDGA . 2011

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