Portal de investigación
Ultrastructural characteristics of human oocytes vitrified before and after in vitro maturation
Fecha de publicación:
Autores de IIS La Fe
Participantes ajenos a IIS La Fe
- Segovia, Y
- Victory, N
- Garcia-Valverde, LM
- Garcia, M
- Aizpurua, J
- Gomez-Torres, MJ
Grupos
Abstract
The development of an effective program that combines in vitro maturation (IVM) and cryopreservation for immature oocytes would represent a novel advance for in vitro fertilization (IVF), especially as a means to preserve the fertility of women in unique situations. The aim of this study was to analyze the ultrastructural characteristics of human oocytes, obtained after controlled ovarian stimulation, to determine whether IVM is best performed before or after vitrification. To this end, we analyzed the following features in a total of 22 MR oocytes: size, zona pellucida and perivitelline space, mitochondria number, M-SER (mitochondria-smooth endoplasmic reticulum) aggregates and M-V (mitochondria-vesicle) complexes, the number of cortical granules and microvilli, and the presence of vacuolization using transmission electron microscopy (TEM). Each oocyte presented a rounded shape, with an intact oolemma, and was surrounded by a continuous zona pellucida and perivitelline space. Statistical analysis comparing oocytes vitrified before or after IVM indicated that there were no significant differences between examined characteristics.
Datos de la publicación
- ISSN/ISSNe:
- 0916-8818, 1348-4400
- Tipo:
- Article
- Páginas:
- 377-382
- DOI:
- 10.1262/jrd.2017-009
- Factor de Impacto:
- 0,725 SCImago ℠
- Cuartil:
- Q1 SCImago ℠
JOURNAL OF REPRODUCTION AND DEVELOPMENT SOCIETY REPRODUCTION & DEVELOPMENT-SRD
Citas Recibidas en Web of Science: 13
Documentos
- No hay documentos
Filiaciones
Keywords
- Cryopreservation; Electron microscopy; In vitro maturation
Proyectos asociados
TRAMIENTO COMBINADO DE VITAMINA D CON AGNRH SOBRE EL CRECIMIENTO DE LOS MIOMAS UTERINOS
Investigador Principal: ANTONIO PELLICER MARTÍNEZ
PI15/00312 . INSTITUTO DE SALUD CARLOS III . 2016
SOFIA: SEGURIDAD DE OVALEAPR (FOLITROPINA ƒ¿) EN MUJERES INFERTILES SOMETIDAS A SUPEROVULACION EN TECNICAS DE REPRODUCCION ASISTIDA. ESTUDIO MULTINACIONAL, COMPARATIVO, PROSPECTIVO, NO INTERVENCIONISTA, DE OBSERVACION DE COHORTES.
Investigador Principal: JOSÉ MARÍA RUBIO RUBIO
TEV-FOL-2016-02 . 2017
ESTUDIO DOBLE CIEGO, ALEATORIZADO, DE FASE 3, CONTROLADO CON PLACEBO PARA EVALUAR LA SEGURIDAD Y EFICACIA DE UNA ÚNICA ADMINISTRACIÓN POR VÍA ORAL DE NOLASIBAN PARA MEJORAR LAS TASAS DE EMBARAZO DESPUÉS DE UN TRATAMIENTO FIV O ICSI EN LOS DÍAS 3 Y 5 DE CICLOS DE TRANSFERENCIA DE EMBRIONES FRESCOS.
Investigador Principal: JOSÉ MARÍA RUBIO RUBIO
16-OBE001-005 . 2017
LUTROPINA ALFA EN MITAD DE LA FASE FOLICULAR EN MUJERES CON RIESGO DE POBRE RESPUESTA EN SUPRESION CON CETRORELIX. ESTUDIO EXPLORATORIO.
Investigador Principal: ALBERTO ROMEU SARRIO
26170 . 2005
Cita
Segovia Y,Victory N,Peinado I,Garcia LM,Garcia M,Aizpurua J,Monzo A,Gomez MJ. Ultrastructural characteristics of human oocytes vitrified before and after in vitro maturation. J. Reprod. Dev. 2017. 63. (4):p. 377-382. IF:1,635. (1).