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  3. Carbapenem-resistant Citrobacter spp. isolated in Spain from 2013 to 2015 produced a variety of carbapenemases including VIM-1, OXA-48, KPC-2, NDM-1 and VIM-2

Carbapenem-resistant Citrobacter spp. isolated in Spain from 2013 to 2015 produced a variety of carbapenemases including VIM-1, OXA-48, KPC-2, NDM-1 and VIM-2

Fecha de publicación:

Autores de IIS La Fe

Participantes ajenos a IIS La Fe

  • Arana, DM
  • Ortega, A
  • Lara, N
  • Bautista, V
  • Saez, D
  • Fernandez-Romero, S
  • Aracil, B
  • Perez-Vazquez, M
  • Campos, J
  • Oteo, J
  • Spanish Antibiotic Resistance Surveillance Programme Collaborating Group

Grupos

Abstract

Objectives: There is little information about carbapenemase-producing (CP) Citrobacter spp. We studied the molecular epidemiology and microbiological features of CP Citrobacter spp. isolates collected in Spain (2013-15). Methods: In total, 119 isolates suspected of being CP by the EUCAST screening cut-off values were analysed. Carbapenemases and ESBLs were characterized using PCR and sequencing. The genetic relationship among Citrobacter freundii isolates was studied by PFGE. Results: Of the 119 isolates, 63 (52.9%) produced carbapenemases, of which 37 (58.7%) produced VIM-1, 20 (31.7%) produced OXA-48, 12 (19%) produced KPC-2, 2 (3.2%) produced NDM-1 and 1 (1.6%) produced VIM2; 9 C. freundii isolates co-produced VIM-1 plus OXA-48. Fourteen isolates (22.2%) also carried ESBLs: 8 CTX-M-9 plus SHV-12, 2 CTX-M-9, 2 SHV-12 and 2 CTX-M-15. Fifty-seven isolates (90.5%) were C. freundii, 4 (6.3%) were Citrobacter koseri, 1 (1.6%) was Citrobacter amalonaticus and 1 (1.6%) was Citrobacter braakii. By EUCAST breakpoints, eight (12.7%) of the CP isolates were susceptible to the four carbapenems tested. In the 53 CP C. freundii analysed by PFGE, a total of 44 different band patterns were observed. Four PFGE clusters were identified: cluster 1 included eight isolates co-producing VIM-1 and OXA-48; bla(VIM-1) was carried in a class 1 integron (intI-bla(VIM-1)-aacA4-dfrB1-aadA1-catB2-qacE Delta 1/sul1) and bla(OXA-48) was carried in a Tn1999.2 transposon. Conclusions: We observed the clonal and polyclonal spread of CP Citrobacter spp. across several Spanish geographical areas. Four species of Citrobacter spp. produced up to five carbapenemase types, including coproduction of VIM-1 plus OXA-48. Some CP Citrobacter spp. isolates were susceptible to the four carbapenems tested, a finding with potential clinical implications.

Datos de la publicación

ISSN/ISSNe:
0305-7453, 1460-2091

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY  OXFORD UNIV PRESS

Tipo:
Article
Páginas:
3283-3287
PubMed:
29029114
Factor de Impacto:
2,419 SCImago ℠
Cuartil:
Q1 SCImago ℠

Citas Recibidas en Web of Science: 49

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