Immunoproteomic studies on paediatric opsoclonus-myoclonus associated with neuroblastoma

Autores de IIS La Fe

• Estefania Torres Vega

  Autor

• Maria Duran Moreno

  Autor

• Yania Yañez Peralta

  Autor

• Adela Cañete Nieto

  Autor

• Victoria Castel Sánchez

  Autor

• Rogelio Alberto Lopez Cuevas

  Autor

• Juan Jesús Vílchez Padilla

  Autor

• Jaime Dalmau Serra

  Autor

• Francesc Graus Ribas

  Autor

• José Manuel García Verdugo

  Autor

• Luís Bataller Alberola

  Autor

Participantes ajenos a IIS La Fe

• Sánchez Del Pino M

Grupos

• Investigación Clínica y Traslacional en Cáncer

• Nutrición y Metabolopatías

• Patología Neuromuscular y Ataxias

Abstract

We aimed to identify new cell-membrane antigens implicated in opsoclonus-myoclonus with neuroblastoma. The sera of 3 out of 14 patients showed IgG electron-microscopy immunogold reactivity on SH-SY5Y neuroblastoma cells. Immunoprecipitation experiments using rat brain synaptosomes and SH-SY5Y cells led to the identification of: (1) thirty-one nuclear/cytoplasmic proteins (including antigens HuB, HuC); (2) seven neuronal membrane proteins, including the Shaw-potassium channel Kv3.3 (KCNO), whose genetic disruption in mice causes ataxia and generalized muscle twitching. Although cell-based assays did not demonstrate direct antigenicity, our findings point to Shaw-related subfamily of the potassium voltage-gated channels complexed proteins as hypothetical antigenic targets. (C) 2016 Elsevier B.V. All rights reserved.

Keywords

• Opsoclonus-myoclonus; Immunoproteomics; Synaptosomes; Potassium channels