Multicenter Study of Epidemiological Cutoff Values and Detection of Resistance in Candida spp. to Anidulafungin, Caspofungin, and Micafungin Using the Sensititre YeastOne Colorimetric Method
Autores de IIS La Fe
Participantes ajenos a IIS La Fe
- Espinel-Ingroff A
- Alvarez-Fernandez M
- Carver PL
- Chen SC
- Eschenauer G
- Getsinger DL
- Gonzalez GM
- Govender NP
- Grancini A
- Hanson KE
- Kidd SE
- Klinker K
- Kubin CJ
- Kus JV
- Lockhart SR
- Meletiadis J
- Morris AJ
- Pelaez T
- Quindós G
- Rodriguez-Iglesias M
- Sánchez-Reus F
- Shoham S
- Wengenack NL
- Borrell Solé N
- Echeverria J
- Esperalba J
- Gómez-G de la Pedrosa E
- García García I
- Linares MJ
- Marco F
- Merino P
- Pérez Del Molino L
- Roselló Mayans E
- Rubio Calvo C
- Ruiz Pérez de Pipaon M
- Yagüe G
- Garcia-Effron G
- Guinea J
- Perlin DS
- Sanguinetti M
- Shields R
- Turnidge J
Grupos
Abstract
Neither breakpoints (BPs) nor epidemiological cutoff values (ECVs) have been established for Candida spp. with anidulafungin, caspofungin, and micafungin when using the Sensititre YeastOne (SYO) broth dilution colorimetric method. In addition, reference caspofungin MICs have so far proven to be unreliable. Candida species wild-type (WT) MIC distributions (for microorganisms in a species/drug combination with no detectable phenotypic resistance) were established for 6,007 Candida albicans, 186 C. dubliniensis, 3,188 C. glabrata complex, 119 C. guilliermondii, 493 C. krusei, 205 C. lusitaniae, 3,136 C. parapsilosis complex, and 1,016 C. tropicalis isolates. SYO MIC data gathered from 38 laboratories in Australia, Canada, Europe, Mexico, New Zealand, South Africa, and the United States were pooled to statistically define SYO ECVs. ECVs for anidulafungin, caspofungin, and micafungin encompassing >= 97.5% of the statistically modeled population were, respectively, 0.12, 0.25, and 0.06 mu g/ml for C. albicans, 0.12, 0.25, and 0.03 mu g/ml for C. glabrata complex, 4, 2, and 4 mu g/ml for C. parapsilosis complex, 0.5, 0.25, and 0.06 mu g/ml for C. tropicalis, 0.25, 1, and 0.25 mu g/ml for C. krusei, 0.25, 1, and 0.12 mu g/ml for C. lusitaniae, 4, 2, and 2 mu g/ml for C. guilliermondii, and 0.25, 0.25, and 0.12 mu g/ml for C. dubliniensis. Species-specific SYO ECVs for anidulafungin, caspofungin, and micafungin correctly classified 72 (88.9%), 74 (91.4%), 76 (93.8%), respectively, of 81 Candida isolates with identified fks mutations. SYO ECVs may aid in detecting non-WT isolates with reduced susceptibility to anidulafungin, micafungin, and especially caspofungin, since testing the susceptibilities of Candida spp. to caspofungin by reference methodologies is not recommended.
Datos de la publicación
- ISSN/ISSNe:
- 0066-4804, 1098-6596
- Tipo:
- Article
- Páginas:
- 6725-6732
- DOI:
- 10.1128/AAC.01250-15
- Factor de Impacto:
- 2,343 SCImago ℠
- Cuartil:
- Q1 SCImago ℠
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY AMER SOC MICROBIOLOGY
Citas Recibidas en Web of Science: 51
Documentos
- No hay documentos
Filiaciones
Proyectos asociados
FUNGEMIA: EPIDEMIOLOGIA, MECANISMOS DE RESISTENCIA ANTIFUNGICA Y TIPADO MOLECULAR
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NUEVAS ESTRATEGIAS PARA EL TRATAMIENTO DE LAS INFECCIONES ASOCIADAS A BIOCAPAS FUNGICAS.
Investigador Principal: EMILIA CANTON LACASA
PI12/02786 . INSTITUTO DE SALUD CARLOS III . 2013
ESTUDIO EPIDEMIOLOGICO DE DIAGNOSTICO PRECOZ DE LA CANDIDIASIS INVASORA EN PACIENTES NEONATOS PRETERMINO DE MUY BAJO PESO. ESTUDIO CANDI-NEO
Investigador Principal: MÁXIMO VENTO TORRES
AST-MYC-2010-01
ESTUDIO OBSERVACIONAL, PROSPECTIVO Y MULTICÉNTRICO EN CUIDADOS INTENSIVOS PARA EVALUAR LA FIABILIDAD DIAGNÓSTICOA DE LA PCR (REACCIÓN EN CADENA DE LA POLIMERASA) EN PACIENTES EN TRATAMIENTO ANTIFÚNGICO EMPÍRICO POR SOSPECHA DE CANDIDIASIS INVASIVA. (ESTUD IO MICAFEM).
Investigador Principal: PAULA RAMÍREZ GALLEYMORE
AST-INF-2012-01
Cita
Espinel A,Alvarez M,Cantón E,Carver PL,Chen SC,Eschenauer G,Getsinger DL,Gonzalez GM,Govender NP,Grancini A,Hanson KE,Kidd SE,Klinker K,Kubin CJ,Kus JV,Lockhart SR,Meletiadis J,Morris AJ,Pelaez T,Quindós G,Rodriguez M,Sánchez F,Shoham S,Wengenack NL,Borrell N,Echeverria J,Esperalba J,Gómez E,García I,Linares MJ,Marco F,Merino P,Pemán J,Pérez Del Molino L,Roselló E,Rubio C,Ruiz Pérez de Pipaon M,Yagüe G,Garcia G,Guinea J,Perlin DS,Sanguinetti M,Shields R,Turnidge J. Multicenter Study of Epidemiological Cutoff Values and Detection of Resistance in Candida spp. to Anidulafungin, Caspofungin, and Micafungin Using the Sensititre YeastOne Colorimetric Method. Antimicrob Agents Chemother. 2015. 59. (11):p. 6725-6732. IF:4,415. (1).