Multicenter Study of Epidemiological Cutoff Values and Detection of Resistance in Candida spp. to Anidulafungin, Caspofungin, and Micafungin Using the Sensititre YeastOne Colorimetric Method

Fecha de publicación:

Autores de IIS La Fe

Participantes ajenos a IIS La Fe

  • Espinel-Ingroff A
  • Alvarez-Fernandez M
  • Carver PL
  • Chen SC
  • Eschenauer G
  • Getsinger DL
  • Gonzalez GM
  • Govender NP
  • Grancini A
  • Hanson KE
  • Kidd SE
  • Klinker K
  • Kubin CJ
  • Kus JV
  • Lockhart SR
  • Meletiadis J
  • Morris AJ
  • Pelaez T
  • Quindós G
  • Rodriguez-Iglesias M
  • Sánchez-Reus F
  • Shoham S
  • Wengenack NL
  • Borrell Solé N
  • Echeverria J
  • Esperalba J
  • Gómez-G de la Pedrosa E
  • García García I
  • Linares MJ
  • Marco F
  • Merino P
  • Pérez Del Molino L
  • Roselló Mayans E
  • Rubio Calvo C
  • Ruiz Pérez de Pipaon M
  • Yagüe G
  • Garcia-Effron G
  • Guinea J
  • Perlin DS
  • Sanguinetti M
  • Shields R
  • Turnidge J

Grupos

Abstract

Neither breakpoints (BPs) nor epidemiological cutoff values (ECVs) have been established for Candida spp. with anidulafungin, caspofungin, and micafungin when using the Sensititre YeastOne (SYO) broth dilution colorimetric method. In addition, reference caspofungin MICs have so far proven to be unreliable. Candida species wild-type (WT) MIC distributions (for microorganisms in a species/drug combination with no detectable phenotypic resistance) were established for 6,007 Candida albicans, 186 C. dubliniensis, 3,188 C. glabrata complex, 119 C. guilliermondii, 493 C. krusei, 205 C. lusitaniae, 3,136 C. parapsilosis complex, and 1,016 C. tropicalis isolates. SYO MIC data gathered from 38 laboratories in Australia, Canada, Europe, Mexico, New Zealand, South Africa, and the United States were pooled to statistically define SYO ECVs. ECVs for anidulafungin, caspofungin, and micafungin encompassing >= 97.5% of the statistically modeled population were, respectively, 0.12, 0.25, and 0.06 mu g/ml for C. albicans, 0.12, 0.25, and 0.03 mu g/ml for C. glabrata complex, 4, 2, and 4 mu g/ml for C. parapsilosis complex, 0.5, 0.25, and 0.06 mu g/ml for C. tropicalis, 0.25, 1, and 0.25 mu g/ml for C. krusei, 0.25, 1, and 0.12 mu g/ml for C. lusitaniae, 4, 2, and 2 mu g/ml for C. guilliermondii, and 0.25, 0.25, and 0.12 mu g/ml for C. dubliniensis. Species-specific SYO ECVs for anidulafungin, caspofungin, and micafungin correctly classified 72 (88.9%), 74 (91.4%), 76 (93.8%), respectively, of 81 Candida isolates with identified fks mutations. SYO ECVs may aid in detecting non-WT isolates with reduced susceptibility to anidulafungin, micafungin, and especially caspofungin, since testing the susceptibilities of Candida spp. to caspofungin by reference methodologies is not recommended.

Datos de la publicación

ISSN/ISSNe:
0066-4804, 1098-6596

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY  AMER SOC MICROBIOLOGY

Tipo:
Article
Páginas:
6725-6732
Factor de Impacto:
2,343 SCImago
Cuartil:
Q1 SCImago

Citas Recibidas en Web of Science: 51

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