Development and application of recombinant antibody-based immunoassays to tetraconazole residue analysis in fruit juices
Autores de IIS La Fe
Participantes ajenos a IIS La Fe
- Manclús JJ
Grupos
Abstract
Tetraconazole is currently used as a fungicide in fruit and vegetables. The aim of this work was the development of immunochemical techniques based on recombinant antibodies for the screening of tetraconazole residues in fruit juices. Recombinant antibodies were produced from a hybridoma cell line secreting a monoclonal antibody specific for tetraconazole and from lymphocytes of mice hyperimmunised with tetraconazole haptens conjugated to bovine serum albumin. From these antibodies, enzyme-linked immunosorbent assays in the conjugate-coated format were developed, which were able to detect tetraconazole standards down to 1 ng/mL. From recovery studies with spiked samples, these immunoassays determined tetraconazole in orange and apple juices with acceptable reproducibility (coefficients of variation below 25%) and recoveries (ranging from 78% to 145%) for a screening technique. The analytical performance of RAb-based immunoassays was fairly similar to that of the MAb-based immunoassays. Due to their simplicity and high sample throughput, the developed recombinant-based immunoassays can be valuable analytical tools for the screening of tetraconazole residues in fruit juices at regulatory levels. (C) 2013 Elsevier Ltd. All rights reserved.
Datos de la publicación
- ISSN/ISSNe:
- 0308-8146, 1873-7072
- Tipo:
- Article
- Páginas:
- 205-213
- PubMed:
- 24054232
- Factor de Impacto:
- 1,557 SCImago ℠
- Cuartil:
- Q1 SCImago ℠
FOOD CHEMISTRY ELSEVIER SCI LTD
Citas Recibidas en Web of Science: 17
Documentos
- No hay documentos
Filiaciones
Keywords
- lmmunochemical analysis; Recombinant antibodies; ScFv; ELISA; Tetraconazole; Fungicide residues; Fruit juices
Proyectos y Estudios Clínicos
ENSAYO FASE IIIB, ALEATORIZADO, DOBLE CIEGO, DE GRUPOS PARALELOS, MULTICÉNTRICO, PARA COMPARAR EL RIESGO DE MUERTE CARDIOVASCULAR, INFARTO DE MIOCARDIO E ICTUS ISQUÉMICO EN PACIENTES CON ENFERMEDAD ARTERIAL PERIFÉRICA ESTABLECIDA, TRATADOS CON TICAGRELOR VS CLOPIDOGREL (EUCLIDEXAMINANDO EL USO DE TICAGRELOR EN EAP).
Investigador Principal: MANUEL MIRALLES HERNÁNDEZ
D5135C00001
Cita
PLANA E,MORENO MJ,MONTOYA Á,Manclús JJ. Development and application of recombinant antibody-based immunoassays to tetraconazole residue analysis in fruit juices. Food Chem. 2014. 143. p. 205-213. IF:3,391. (1).