The association of thromboxane A(2) receptor with lipid rafts is a determinant for platelet functional responses

Autores de IIS La Fe

• Antonio Moscardó Martínez

  Autor

• Juana Valles Giner

  Autor

• Ana María Latorre Campos

  Autor

• Maria Teresa Santos Diaz

  Autor

Grupos

• Hemostasia, Trombosis, Arteriosclerosis y Biología vascular

Abstract

We have investigated the presence of thromboxane A(2) (TXA(2)) receptor associated with lipid rafts in human platelets and the regulation of platelet function in response to TXA(2) receptor agonists when lipid rafts are disrupted by cholesterol extraction. Platelet aggregation with TXA(2) analogs U46619 and IBOP was almost blunted in cholesterol-depleted platelets, as well as alpha(IIb)beta(3) integrin activation and P-selectin exposure. Raft disruption also inhibited TXA(2)-induced cytosolic calcium increase and nucleotide release, ruling out an implication of P2Y(12) receptor. An important proportion of TXA(2) receptor (40%) was colocalized at lipid rafts. The presence of the TXA(2) receptor associated with lipid rafts in platelets is important for functional platelet responses to TXA(2). (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Keywords

• Lipid raft; Platelet; Signal transduction; Thromboxane A(2); Thromboxane receptor