Nanoemulsion Increases the Antifungal Activity of Amphotericin B against Four Candida auris Clades: In Vitro and In Vivo Assays.

Fecha de publicación: 21/06/2023 Fecha Ahead of Print: 21/06/2023

Autores de IIS La Fe

Alba Cecilia Ruiz Gaitán

Autor
Víctor García Bustos

Autor
María Ángeles Tormo Mas

Autor
José Manuel Pérez Royo

Autor
Verónica Patricia Bernabé Quispe

Autor
María Dolores Pérez Ruiz

Autor
Lara Zaragoza Macian

Autor
Carmen Vicente Saez

Autor
Eulogio Valentín Gómez

Autor
Javier Pemán García

Autor

Participantes ajenos a IIS La Fe

Marena, Gabriel Davi
Lopez, Alejandro
Avalos Mansilla, Antonia
Carvalho, Gabriela Correa
Bauab, Tais Maria
Chorilli, Marlus

Grupos

Infección Grave

Investigador Principal

María Ángeles Tormo Mas

Abstract

Candida auris is an emerging yeast of worldwide interest due to its antifungal resistance and mortality rates. The aim of this study was to analyse the in vitro and in vivo antifungal activity of a nanoemulsion loaded with amphotericin B (NEA) against planktonic cells and biofilm of C. auris clinical isolates belonging to four different clades. In vivo assays were performed using the Galleria mellonella model to analyse antifungal activity and histopathological changes. The in vitro results showed that NEA exhibited better antifungal activity than free amphotericin B (AmB) in both planktonic and sessile cells, with >31% inhibition of mature biofilm. In the in vivo assays, NEA demonstrated superior antifungal activity in both haemolymph and tissue. NEA reduced the fungal load in the haemolymph more rapidly and with more activity in the first 24 h after infection. The histological analysis of infected larvae revealed clusters of yeast, immune cells, melanisation, and granulomas. In conclusion, NEA significantly improved the in vitro and in vivo antifungal activity of AmB and could be considered a promising therapy for C. auris infections.

Datos de la publicación

ISSN/ISSNe:: 2076-2607, 2076-2607
Tipo:: Article
Páginas:: -
DOI:: 10.3390/microorganisms11071626
Factor de Impacto:: 0,862 SCImago ℠
Cuartil:: Q2 SCImago ℠

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Métricas

Filiaciones

Marena, Gabriel Davi:: 1Severe Infection Research Group, Health Research Institute La Fe, 46026 Valencia, Spain.

2Department of Drugs and Medicines, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara 14800-903, Brazil.

3Department of Biological Sciences, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara 14800-903, Brazil.
Ruiz-Gaitan, Alba:: 1Severe Infection Research Group, Health Research Institute La Fe, 46026 Valencia, Spain.

4Department of Medical Microbiology, University and Polytechnic La Fe Hospital, 46026 Valencia, Spain.
Garcia-Bustos, Victor:: 1Severe Infection Research Group, Health Research Institute La Fe, 46026 Valencia, Spain.

5Department of Pathology, Faculty of Medicine and Dentistry, University of Valencia, 46010 Valencia, Spain.
Tormo-Mas, Maria Angeles:: 1Severe Infection Research Group, Health Research Institute La Fe, 46026 Valencia, Spain.
Perez-Royo, Jose Manuel:: 1Severe Infection Research Group, Health Research Institute La Fe, 46026 Valencia, Spain.
Lopez, Alejandro:: 1Severe Infection Research Group, Health Research Institute La Fe, 46026 Valencia, Spain.
Bernarbe, Patricia:: 1Severe Infection Research Group, Health Research Institute La Fe, 46026 Valencia, Spain.
Perez Ruiz, Maria Dolores:: 6Department of Pathological Anatomy, La Fe Hospital, 46026 Valencia, Spain.
Zaragoza Macian, Lara:: 6Department of Pathological Anatomy, La Fe Hospital, 46026 Valencia, Spain.
Vicente Saez, Carmen:: 6Department of Pathological Anatomy, La Fe Hospital, 46026 Valencia, Spain.
Gomez, Eulogio Valentin:: 1Severe Infection Research Group, Health Research Institute La Fe, 46026 Valencia, Spain.

7Department of Microbiology and Ecology, University of Valencia, 46010 Valencia, Spain.
Carvalho, Gabriela Correa:: 2Department of Drugs and Medicines, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara 14800-903, Brazil.

3Department of Biological Sciences, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara 14800-903, Brazil.
Bauab, Tais Maria:: 3Department of Biological Sciences, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara 14800-903, Brazil.
Chorilli, Marlus:: 2Department of Drugs and Medicines, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara 14800-903, Brazil.
Peman, Javier:: 1Severe Infection Research Group, Health Research Institute La Fe, 46026 Valencia, Spain.

4Department of Medical Microbiology, University and Polytechnic La Fe Hospital, 46026 Valencia, Spain.

Keywords

Candida auris; Galleria mellonella; amphotericin B; infectious disease; nanoemulsion

Proyectos asociados

FUNGEMIA: EPIDEMIOLOGIA, MECANISMOS DE RESISTENCIA ANTIFUNGICA Y TIPADO MOLECULAR

090/2006- EVES - PEMAN . 2006

NUEVAS ESTRATEGIAS PARA EL TRATAMIENTO DE LAS INFECCIONES ASOCIADAS A BIOCAPAS FUNGICAS.

Investigador Principal: EMILIA CANTON LACASA

PI12/02786 . INSTITUTO DE SALUD CARLOS III . 2013

SOLICITUD DE CENTRO I+D RAMON Y CAJAL

Investigador Principal: MARÍA ÁNGELES TORMO MAS

RYC-2012-12246 . MINISTERIO DE ECONOMIA Y COMPETITIVIDAD; FUNDACIÓN PARA LA INVESTIGACIÓN DEL HOSPITAL UNIVERSITARIO LA FE DE LA COMUNIDAD VALENCIANA . 2014

MICROBIAL COMPARATIVE GENOMICS-MICROGEN

Investigador Principal: MARÍA ÁNGELES TORMO MAS

CSD2009-00006_CONSOLIDER . MINISTERIO DE ECONOMIA Y COMPETITIVIDAD; FUNDACIÓN PARA LA INVESTIGACIÓN DEL HOSPITAL UNIVERSITARIO LA FE DE LA COMUNIDAD VALENCIANA . 2009