Portal de investigación
High-Capacity Mesoporous Silica Nanocarriers of siRNA for Applications in Retinal Delivery.
Fecha de publicación:
Fecha Ahead of Print:
Autores de IIS La Fe
Participantes ajenos a IIS La Fe
- Ultimo, Amelia
- Orzaez, Mar
- Santos-Martinez, Maria J.
- Marcos, Maria D.
- Ruiz-Hernandez, Eduardo
Grupos
Abstract
The main cause of subretinal neovascularisation in wet age-related macular degeneration (AMD) is an abnormal expression in the retinal pigment epithelium (RPE) of the vascular endothelial growth factor (VEGF). Current approaches for the treatment of AMD present considerable issues that could be overcome by encapsulating anti-VEGF drugs in suitable nanocarriers, thus providing better penetration, higher retention times, and sustained release. In this work, the ability of large pore mesoporous silica nanoparticles (LP-MSNs) to transport and protect nucleic acid molecules is exploited to develop an innovative LP-MSN-based nanosystem for the topical administration of anti-VEGF siRNA molecules to RPE cells. siRNA is loaded into LP-MSN mesopores, while the external surface of the nanodevices is functionalised with polyethylenimine (PEI) chains that allow the controlled release of siRNA and promote endosomal escape to facilitate cytosolic delivery of the cargo. The successful results obtained for VEGF silencing in ARPE-19 RPE cells demonstrate that the designed nanodevice is suitable as an siRNA transporter.
Datos de la publicación
- ISSN/ISSNe:
- 1661-6596, 1661-6596
- Tipo:
- Article
- Páginas:
- -
- DOI:
- 10.3390/ijms24032753
- PubMed:
- 36769075
- Factor de Impacto:
- 1,176 SCImago ℠
- Cuartil:
- Q1 SCImago ℠
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES MDPI AG
Citas Recibidas en Web of Science: 2
Documentos
- No hay documentos
Filiaciones
Keywords
- VEGF silencing; age-related macular degeneration; large pore mesoporous silica nanoparticles; siRNA delivery
Proyectos y Estudios Clínicos
NANOMATERIALES INTELIGENTES, SONDAS Y DISPOSITIVOS PARA EL DESARROLLO INTEGRADO DE NUEVAS HERRAMIENTAS APLICADAS AL CAMPO BIOMÉDICO
Investigador Principal: JUAN BAUTISTA SALOM SANVALERO
MAT2015-64139-C4-1-R . MINISTERIO DE ECONOMIA Y COMPETITIVIDAD . 2016
PROGRAMA TÉCNICO DE APOYO. MODALIDAD INFRAESTRUCTURA.
Investigador Principal: RAMÓN MARTÍNEZ MÁÑEZ
PTA2016-12603-1 . MINISTERIO DE ECONOMIA Y COMPETITIVIDAD . 2018
PLATAFORMA PARA LA DETECCIÓN DE PATÓGENOS BASADA EN MATERIALES CON PUERTAS MOLECULARES (PATH-GATE).
Investigador Principal: RAMÓN MARTÍNEZ MÁÑEZ
DTS18/00090 . INSTITUTO DE SALUD CARLOS III . 2019
Contrato i-PFIS Doctorados IIS - empresa en Ciencias y Tecnologías de la Salud.
Investigador Principal: FÉLIX SANCENÓN GALARZA
IFI19/00026 . INSTITUTO DE SALUD CARLOS III . 2020
Contratos Sara Borrell 2020. DIEZ SÁNCHEZ, PAULA
Investigador Principal: FÉLIX SANCENÓN GALARZA
CD20/00120 . INSTITUTO DE SALUD CARLOS III . 2021
Cita
Ultimo A,Orzaez M,Santos MJ,MARTINEZ R,Marcos MD,SANCENON F,Ruiz E. High-Capacity Mesoporous Silica Nanocarriers of siRNA for Applications in Retinal Delivery. Int. J. Mol. Sci. 2023. 24. (3):2753. IF:4,900. (1).