Usefulness of Chromogenic Media with Fluconazole Supplementation for Presumptive Identification of Candida auris.

Fecha de publicación: 08/01/2023 Fecha Ahead of Print: 08/01/2023

Autores de IIS La Fe

Alba Cecilia Ruiz Gaitán

Autor
Ignacio Antonio Sigona Giangreco

Autor
José Manuel Pérez Royo

Autor
Víctor García Bustos

Autor
Salvador Giner Almaraz

Autor
Javier Pemán García

Autor

Participantes ajenos a IIS La Fe

Marta García Hita
Valentin-Gomez, Eulogio
de Groot, Piet W. J.

Grupos

Infección Grave

Investigador Principal

María Ángeles Tormo Mas

Abstract

Introduction:Candida auris is a major threat to public health. Rapid detection is essential for early treatment and transmission control. The use of chromogenic media allows the presumptive identification of this new species. The aim of this study is to describe the morphological characteristics of C. auris colonies on three commercial chromogenic media. Methods: Nineteen C. auris isolates from different countries/clades and 18 isolates of other species were cultivated in CHROMagar(TM) Candida Plus, HiCrome(TM) Candida, CHROMagar-Candida, and fluconazole-supplemented (32 mg/L) CHROMagar-Candida media. Results: On CHROMagar(TM) Candida Plus and HiCrome(TM) Candida, C. auris isolates from Colombia, Venezuela, India, Korea, and Japan displayed blue-shaded colonies, while isolates from Spain and Germany exhibited light pink shades with a bluish halo. All isolates showed white to pink colonies on CHROMagar-Candida. On CHROMagar Candida supplemented with fluconazole, whilst C. auris, C. glabrata, or C. krusei showed a similar pink color at 48 h incubation, phenotypic differentiation was possible by the rough, paraffin-like texture or the intense purple color acquired by C. krusei and C. glabrata, respectively. Moreover, in this medium, the presence of C. auris in combination with other species of similar color was not limiting for its early identification, due to this medium selecting only strains resistant to this antifungal. Conclusions: The use of chromogenic media such as CHROMagar(TM) Candida Plus facilitates a presumptive identification of C. auris. However, this identification can be difficult in the presence of mixed cultures. In these cases, the use of CHROMagar(TM) Candida medium with 32 mg/L fluconazole offers better performance for the identification of C. auris by inhibiting fluconazole-susceptible strains and selecting rare or high fluconazole MIC (>32 mg/L) isolates.

Datos de la publicación

ISSN/ISSNe:: 2075-4418, 2075-4418
Tipo:: Article
Páginas:: -
DOI:: 10.3390/diagnostics13020231
Factor de Impacto:: 0,658 SCImago ℠
Cuartil:: Q2 SCImago ℠

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Filiaciones

Ruiz-Gaitan, Alba:: 1 La Fe Univ & Polytech Hosp, Dept Clin Microbiol, Valencia 46026, Spain

2 Med Res Inst La Fe, Severe Infect Res Grp, Valencia 46026, Spain
Sigona-Giangreco, Ignacio:: 2 Med Res Inst La Fe, Severe Infect Res Grp, Valencia 46026, Spain
Perez-Royo, Jose Manuel:: 2 Med Res Inst La Fe, Severe Infect Res Grp, Valencia 46026, Spain
Garcia-Bustos, Victor:: 2 Med Res Inst La Fe, Severe Infect Res Grp, Valencia 46026, Spain

3 La Fe Univ & Polytech Hosp, Dept Internal Med, Valencia 46026, Spain
:: 5 Univ Castilla La Mancha, Reg Ctr Biomed Res, Castilla La Mancha Sci & Technol Pk, Albacete 02008, Spain
Valentin-Gomez, Eulogio:: 4 Univ Valencia, Dept Microbiol & Ecol, Valencia 46010, Spain
Almaraz, Salvador Giner:: 1 La Fe Univ & Polytech Hosp, Dept Clin Microbiol, Valencia 46026, Spain
de Groot, Piet W. J.:: 5 Univ Castilla La Mancha, Reg Ctr Biomed Res, Castilla La Mancha Sci & Technol Pk, Albacete 02008, Spain
Peman, Javier:: 1 La Fe Univ & Polytech Hosp, Dept Clin Microbiol, Valencia 46026, Spain

2 Med Res Inst La Fe, Severe Infect Res Grp, Valencia 46026, Spain