The Maternal Cytokine and Chemokine Profile of Naturally Conceived Gestations Is Mainly Preserved during In Vitro Fertilization and Egg Donation Pregnancies

Autores de IIS La Fe

• Alicia Martínez Varea

  Autor

• David Pellicer Bossis

  Autor

• Vicente Serra Serra

  Autor

• David Hervás Marín

  Autor

• Alicia Martinez Romero

  Autor

• José Bellver Pradas

  Autor

• Alfredo José Perales Marín

  Autor

• Antonio Pellicer Martínez

  Autor

Grupos

• Hematología y Hemoterapia

• Medicina Fetal y Reproductiva

Abstract

This prospective longitudinal study aimed at comparing maternal immune response among naturally conceived (NC; n = 25), in vitro fertilization (IVF; n = 25), and egg donation (ED; n = 25) pregnancies. The main outcome measures were, firstly, to follow up plasma levels of interleukin (IL) 1beta, IL2, IL4, IL5, IL6, IL8, IL10, IL17, interferon gamma, tumor necrosis factor-alpha (TNF alpha), transforming growth factor-beta (TGF beta), regulated upon activation normal T-cell expressed and secreted (RANTES), stromal cell-derived factor 1 alpha (SDF1 alpha), and decidual granulocyte-macrophage colony-stimulating factor (GM-CSF) during the three trimesters of pregnancy during the three trimesters of pregnancy; secondly, to evaluate if the cytokine and chemokine pattern of ED pregnant women differs from that of those with autologous oocytes and, thirdly, to assess if women with preeclampsia show different cytokine and chemokine profile throughout pregnancy versus women with uneventful pregnancies. Pregnant women in the three study groups displayed similar cytokine and chemokine pattern throughout pregnancy. The levels of all quantified cytokines and chemokines, except RANTES, TNF alpha, IL8, TGF beta, and SDF1 alpha, rose in the second trimester compared with the first, and these higher values remained in the third trimester. ED pregnancies showed lower SDF1 alpha levels in the third trimester compared with NC and IVF pregnancies. Patients who developed preeclampsia displayed higher SDF1 alpha plasma levels in the third trimester.

Keywords

• REGULATORY T-CELLS; TROPHOBLAST INVASION; PERIPHERAL-BLOOD; PREECLAMPSIA; TOLERANCE; ANGIOGENESIS; MODULATION; EXPRESSION; INTERFACE; INDUCTION