IVF/ICSI cumulative live birth rates per consumed oocyte remain comparable regardless of sperm DNA fragmentation by TUNEL.

Fecha de publicación: 01/06/2022 Fecha Ahead of Print: 23/02/2022

Autores de IIS La Fe

Participantes ajenos a IIS La Fe

Gil Julia M

Grupos

Biología Reproductiva y Bioingeniería en Reproducción Humana

Leader

Nicolás Garrido Puchalt

Abstract

RESEARCH QUESTION: Does sperm DNA fragmentation (SDF) affect reproductive success of IVF and intracytoplasmic sperm injection (ICSI) cycles measured as cumulative live birth rates (CLBR) in unselected couples? DESIGN: Clinical data from 1339 couples undergoing 2759 IVF/ICSI cycles using autologous oocytes with a SDF test by TdT (terminal deoxynucleotidyl transferase)-mediated dUDP nick-end labelling (TUNEL) assay on their ejaculated spermatozoa were retrospectively evaluated. Main outcomes were calculated according to two different analyses: using 15% SDF as cut-off point (low =15% and high >15%); and categorizing participants based on four SDF ranges (<10%, 10- <20%, 20-30% and >30%). Live birth rate and CLBR per number of embryo transfers, per number of embryos replaced and consumed oocytes required to achieve the first live birth according to level of SDF were the main outcomes assessed. RESULTS: No significant difference was found in clinical pregnancy rate and miscarriage rate between both groups. No differences in LBR per embryo transfer were found for the first or for all embryo transfers when comparing =15% and >15% sperm DNA fragmentation or by SDF ranges. The CLBR according to the number of embryo transfers and the number of embryos replaced showed no statistically significant differences between different SDF groups. When the same number of oocytes were inseminated, similar CLBR were obtained regardless of the degree of male sperm DNA fragmentation. CONCLUSIONS: High SDF did not impair live birth rates of unselected males undergoing IVF/ICSI cycles with autologous oocytes per transfer or the cumulative probability of a live birth.

Datos de la publicación

ISSN/ISSNe:: 1472-6483, 1472-6491
Tipo:: Article
Páginas:: 1079-1089
DOI:: 10.1016/j.rbmo.2022.02.010
Factor de Impacto:: 1,171 SCImago ℠
Cuartil:: Q1 SCImago ℠

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Filiaciones

Hervas, Irene:: Instituto de Investigación. IVI Foundation, Instituto de Investigación Sanitaria La Fe (IIS La Fe), Avenida Fernando Abril Martorell, 106 - Torre A, Planta 1ª, Valencia 46026, Spain
Pacheco, Alberto:: Andrology Laboratory and Sperm Bank, IVIRMA Madrid, Av. del Talgo 68-70, Madrid 28023, Spain

Alfonso X 'El Sabio' University, Avenida de la Universidad 1, Villanueva de la Cañada, Madrid 28691, Spain
Rivera-Egea, Rocio:: Andrology Laboratory and Sperm Bank, IVIRMA Valencia, Plaza de la Policia Local 3, Valencia 46015, Spain
Gil Julia M:: Instituto de Investigación. IVI Foundation, Instituto de Investigación Sanitaria La Fe (IIS La Fe), Avenida Fernando Abril Martorell, 106 - Torre A, Planta 1ª, Valencia 46026, Spain
Navarro-Gomezlechon, Ana:: Instituto de Investigación. IVI Foundation, Instituto de Investigación Sanitaria La Fe (IIS La Fe), Avenida Fernando Abril Martorell, 106 - Torre A, Planta 1ª, Valencia 46026, Spain
Garrido, Nicolas:: Instituto de Investigación. IVI Foundation, Instituto de Investigación Sanitaria La Fe (IIS La Fe), Avenida Fernando Abril Martorell, 106 - Torre A, Planta 1ª, Valencia 46026, Spain