Novel Chromogenic Medium CHROMagar TM Candida Plus for Detection of Candida auris and Other Candida Species from Surveillance and Environmental Samples: A Multicenter Study.

Fecha de publicación: 09/03/2022 Fecha Ahead of Print: 09/03/2022

Autores de IIS La Fe

Amparo Valentín Martín

Autor
Javier Pemán García

Autor

Participantes ajenos a IIS La Fe

Mulet Bayona, Juan Vicente
Salvador Garcia, Carme
Tormo Palop, Nuria
Gonzalez Padron, Carmelo
Colomina Rodriguez, Javier
Gimeno Cardona, Concepcion

Grupos

Infección Grave

Investigador Principal

María Ángeles Tormo Mas

Abstract

Epidemiological trends show a dramatic increase in the prevalence of fungal infections, and in the isolation of multidrug-resistant species, such as Candida auris. CHROMagar TM Candida (CC; CHROMagar, Paris, France) and other chromogenic media, which are widely used in the clinical laboratory because they allow a rapid identification of most Candida species. Recently, CHROMagar TM Candida Plus (CC-Plus; CHROMagar, Paris, France) was developed to detect and differentiate C. auris in addition to other major clinical Candida species, such as C. albicans , C . tropicalis , C. glabrata , or C. krusei. C. auris colonies display a differential light blue color with a blue halo. A multicentric study was designed to evaluate the performance of the CC-Plus medium in the detection of Candida species in patients' surveillance and environmental samples from three Spanish hospitals with active C. auris outbreaks. A total of 364 patients' surveillance samples and 212 environmental samples were tested. Samples were inoculated in CC and CC-Plus in parallel, and the plates were read at 24 and 48 h. All recovered colonies were presumptively identified according to colony color described by manufacturer, and the definitive identification was performed by mass spectrometry at 48 h. A total of 134 C. auris isolates were obtained (101 from patients' surveillance samples, and 33 from environmental samples). Sensitivity, specificity, and predictive positive and negative values were 99.5%, 100%, 100%, and 99.1%, respectively, for the main clinical Candida species, showing that CC-Plus is comparable to CC, with the advantage of being able to differentiate C. auris from C. parapsilosis . Furthermore, CC-Plus was able to detect one C. albicans , one C. glabrata , and eight C. auris that did not grow in CC. Additionally, the yeast colonies were generally larger, suggesting that this novel medium could be a richer medium, and suitable for surveillance and environmental cultures of C. auris and other clinically relevant Candida species.

Datos de la publicación

ISSN/ISSNe:: 2309-608X, 2309-608X
Tipo:: Article
Páginas:: -
DOI:: 10.3390/jof8030281
Factor de Impacto:: 0,980 SCImago ℠
Cuartil:: Q1 SCImago ℠

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Filiaciones

Mulet Bayona, Juan Vicente:: Department of Microbiology and Parasitology, Consorcio Hospital General Universitario de Valencia, 46014 Valencia, Spain
Salvador Garcia, Carme:: Department of Microbiology and Parasitology, Consorcio Hospital General Universitario de Valencia, 46014 Valencia, Spain
Tormo Palop, Nuria:: Department of Microbiology and Parasitology, Consorcio Hospital General Universitario de Valencia, 46014 Valencia, Spain
Valentin Martin, Amparo:: Department of Microbiology and Parasitology, Hospital Universitari I Politècnic La Fe de Valencia, 46026 Valencia, Spain
Gonzalez Padron, Carmelo:: Department of Microbiology and Parasitology, Hospital Clínico Universitario de Valencia, 46010 Valencia, Spain
Colomina Rodriguez, Javier:: Department of Microbiology and Parasitology, Hospital Clínico Universitario de Valencia, 46010 Valencia, Spain
Peman, Javier:: Department of Microbiology and Parasitology, Hospital Universitari I Politècnic La Fe de Valencia, 46026 Valencia, Spain
Gimeno Cardona, Concepcion:: Department of Microbiology and Parasitology, Consorcio Hospital General Universitario de Valencia, 46014 Valencia, Spain

Department of Microbiology and Ecology, University of Valencia, 46010 Valencia, Spain