MicroRNA-185-5p: a marker of brain-sparing in foetuses with late-onset growth restriction.

Autores de IIS La Fe

• 

  José Morales Roselló

  Autor

• Gabriela Alejandra Loscalzo

  Autor

• Antonio José Cañada Martinez

  Autor

• Alfredo José Perales Marín

  Autor

Participantes ajenos a IIS La Fe

• García-Lopez EM
• Ibañez Cabellos JS
• García-Gimenez JL

Grupos

• Medicina Fetal y Reproductiva

Abstract

To compare the expression of microRNA-185-5p (miR-185-5p) in normal foetuses and in foetuses with late-onset growth restriction (FGR) and to determine the factors influencing this expression. In a prospective study, 40 foetuses (22 of them with late-onset FGR and 18 with normal growth) were scanned with Doppler ultrasound after week 35 and followed until birth. Subsequently, blood samples from umbilical cords were collected after delivery to evaluate the expression of miR-185-5p using real-time qPCR. Finally, multivariable regression analysis was applied to determine the clinical and ultrasonographic factors influencing miR-185-5p expression in both normal and late-onset FGR foetuses. In comparison with normal foetuses, late-onset FGR foetuses expressed upregulation of miR-185-5p (2.26 ± 1.30 versus 1.27 ± 1.03 2^-ddCt, P = 0.011). Multivariable regression analysis confirmed that cerebroplacental ratio (P < 0.05) was the only determinant of this overexpression. FGR foetuses overexpress miR-185-5p in relation to brain-sparing. Future studies will be needed to investigate the role of miR-185 in the management of late-onset FGR.

Keywords

• MicroRNA-185-5p; foetal Doppler; foetal growth; late-onset foetal growth restriction