Genome-wide DNA methylation profiling in anorexia nervosa discordant identical twins.

Fecha de publicación: 10/01/2022 Fecha Ahead of Print: 10/01/2022

Autores de IIS La Fe

Carmen Iranzo Tatay

Autor
Luis Miguel Rojo Bofill

Autor
Diana Garcia Latorre

Autor
Inés Calabria Torres

Autor
Juan Silvestre Oltra Soler

Autor
Juan Sandoval Del Amor

Autor
Luis Miguel Rojo Moreno

Autor

Participantes ajenos a IIS La Fe

Hervas-Marin, D.
Vaz-Leal, F. J.
Beato-Fernandez, L.

Grupos

Abstract

Up until now, no study has looked specifically at epigenomic landscapes throughout twin samples, discordant for Anorexia nervosa (AN). Our goal was to find evidence to confirm the hypothesis that epigenetic variations play a key role in the aetiology of AN. In this study, we quantified genome-wide patterns of DNA methylation using the Infinium Human DNA Methylation EPIC BeadChip array ("850 K") in DNA samples isolated from whole blood collected from a group of 7 monozygotic twin pairs discordant for AN. Results were then validated performing a genome-wide DNA methylation profiling using DNA extracted from whole blood of a group of non-family-related AN patients and a group of healthy controls. Our first analysis using the twin sample revealed 9 CpGs associated to a gene. The validation analysis showed two statistically significant CpGs with the rank regression method related to two genes associated to metabolic traits, PPP2R2C and CHST1. When doing beta regression, 6 of them showed statistically significant differences, including 3 CpGs associated to genes JAM3, UBAP2L and SYNJ2. Finally, the overall pattern of results shows genetic links to phenotypes which the literature has constantly related to AN, including metabolic and psychological traits. The genes PPP2R2C and CHST1 have both been linked to the metabolic traits type 2 diabetes through GWAS studies. The genes UBAP2L and SYNJ2 have been related to other psychiatric comorbidity.

Datos de la publicación

ISSN/ISSNe:: 2158-3188, 2158-3188
Tipo:: Article
Páginas:: 15-15
DOI:: 10.1038/s41398-021-01776-y
Factor de Impacto:: 2,076 SCImago ℠
Cuartil:: Q1 SCImago ℠

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Métricas

Filiaciones

Iranzo-Tatay, C.:: Psychiatry Service, Hospital la Fe, Valencia, Spain

Department of Psychiatry, Medicine School, University of Valencia, Valencia, Spain
Hervas-Marin, D.:: Department of Applied Statistics and Operational Research and Quality, Universitat Politècnica de València, Valencia, Spain
Rojo-Bofill, L. M.:: Psychiatry Service, Hospital la Fe, Valencia, Spain
Garcia, D.:: Instituto de Investigación. Epigenomics Unit, Instituto de Investigación Sanitaria La Fe, Valencia, Spain
Vaz-Leal, F. J.:: Department of Psychiatry, Medicine School, University of Extremadura, Badajoz, Spain
Calabria, I:: Instituto de Investigación. Epigenomics Unit, Instituto de Investigación Sanitaria La Fe, Valencia, Spain
Beato-Fernandez, L.:: Eating Disorders and Children's Psychiatry Department, Hospital General, Ciudad Real, Spain
Oltra, S.:: Genetics and Prenatal Diagnosis Unit, Hospital La fe, Valencia, Spain
Sandoval, J.:: Instituto de Investigación. Epigenomics Unit, Instituto de Investigación Sanitaria La Fe, Valencia, Spain.

Instituto de Investigación. Biomarkers and Precision Medicine Unit (UByMP), Instituto de Investigación Sanitaria La Fe, Valencia, Spain.
Rojo-Moreno, L.:: Psychiatry Service, Hospital la Fe, Valencia, Spain

Department of Psychiatry, Medicine School, University of Valencia, Valencia, Spain

Consorcio de Investigación Biomédica en Red de Epidemiología y Salud Pública (CIBERESP), Madrid, Spain