Microbiological assessment of arterial allografts processed in a tissue bank

Fecha de publicación: 01/09/2021 Fecha Ahead of Print: 01/09/2021

Autores de IIS La Fe

Javier Pemán García

Autor

Participantes ajenos a IIS La Fe

Mirabet, V.
Arrebola, M.
Briones, J.
Bosch, M. P.
Ocete, M. D.
Melero, A.
Mirabet, S.
Aranda, L.
Guillot, A. J.
Larrea, L. R.
Escobedo-Lucea, C.

Grupos

Infección Grave

Investigador Principal

María Ángeles Tormo Mas

Abstract

The transmission of microbial infection through tissue allografts is one of the main risks that must be controlled in tissue banks. Therefore, microbiological monitoring controls and validated protocols for the decontamination of tissues during processing have been implemented. This study is based on the evaluation of data from microbiological cultures of arteries (mainly long peripheral arteries) processed in the tissue bank of Valencia (Spain). Donors' profile, pre- and post-disinfection tissue samples were assessed. The presence of residual antibiotics in disinfected tissues was determined and the antimicrobial potential of these tissues was tested. Our overall contamination rate was 23.69%, with a disinfection rate (after antibiotic incubation) of 87.5%. Most (76.09%) of the microbial contaminants were identified as Gram positive. Arterial allografts collected from body sites affected by prior organ removal showed higher risk of contamination. Only vancomycin was detected as tissue release. The antimicrobial effect on Candida albicans was lower than that for bacterial species. Risk assessment for microbial contamination suggested the donor's skin and the environment during tissue collection as the main sources for allograft contamination. Antibiotic-disinfected arterial allografts showed antimicrobial potential.

Datos de la publicación

ISSN/ISSNe:: 1389-9333, 1573-6814
Tipo:: Article
Páginas:: 539-549
DOI:: 10.1007/s10561-021-09951-7
Factor de Impacto:: 0,346 SCImago ℠
Cuartil:: Q3 SCImago ℠

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Métricas

Filiaciones

Mirabet, V.:: 1 Cell and Tissue Bank, Centro de Transfusión de la Comunidad Valenciana, Avenida del Cid, 65-A, 46014, Valencia, Spain.
Arrebola, M.:: 2 Service of Vascular Surgery, Hospital Universitario y Politécnico La Fe, Valencia, Spain.
Briones, J.:: 3 Service of Vascular Surgery, Hospital General, Castellón, Spain.
Bosch, M. P.:: 4 Service of Microbiology, Hospital Universitario y Politécnico La Fe, Valencia, Spain.
Ocete, M. D.:: 5 Service of Microbiology, Consorcio Hospital General Universitario, Valencia, Spain.
Melero, A.:: 6 Department of Pharmacy, Pharmaceutical Technology and Parasitology, Universitat de València, Valencia, Spain.
Mirabet, S.:: 7 Laboratory of Biomedical and Tisular Engineering, Fundación de Investigación Hospital General Universitario, Valencia, Spain.
Aranda, L.:: 5 Service of Microbiology, Consorcio Hospital General Universitario, Valencia, Spain.
Guillot, A. J.:: 6 Department of Pharmacy, Pharmaceutical Technology and Parasitology, Universitat de València, Valencia, Spain.
Larrea, L. R.:: 8 Cell and Tissue Bank, Centro de Transfusión de la Comunidad Valenciana, Avenida del Cid, 65-A, 46014, Valencia, Spain.
Escobedo-Lucea, C.:: 7 Laboratory of Biomedical and Tisular Engineering, Fundación de Investigación Hospital General Universitario, Valencia, Spain.
Peman, J.:: 4 Service of Microbiology, Hospital Universitario y Politécnico La Fe, Valencia, Spain.