Timing of spermatogonial stem cell transplantation affects the spermatogenic recovery outcome in mice.

Fecha de publicación: 01/01/2021 Fecha Ahead of Print: 01/01/2021

Autores de IIS La Fe

Jose Vicente Medrano Plaza

Autor
Ana Navarro Gómez-Lechón

Autor
Antonio Pellicer Martínez

Autor
María Del Mar Andrés Moreno

Autor
Edurne Novella Maestre

Autor

Participantes ajenos a IIS La Fe

Acimovic I
Noguera I

Grupos

Biología Reproductiva y Bioingeniería en Reproducción Humana

Leader

Nicolás Garrido Puchalt
Investigación Clínica y Traslacional en Cáncer

Leader

José Gómez Codina
Medicina Reproductiva

Investigador Principal

José Morales Roselló

Abstract

Spermatogonial stem cell transplantation (SSCT) is a strategy that has demonstrated to be feasible to restore spermatogenesis in animal models when it is performed shortly after the gonadotoxic onset to destroy their endogenous germ cells. However, in the case of boys subjected to fertility preservation, future transplantations will be performed with a delay of many years. In order to study how timing of SSCT affects donor-derived spermatogenic recovery in mice, we compared the percentage of spermatogenic tubule cross-sections within testes of 59 C57BL/6NCrl mice distributed in 6 groups: group 1, untreated mice controls (n = 9); group 2, mice that received a single dose of busulfan 40 mg/kg (n = 10); group 3, mice that received two additional doses of busulfan 10 mg/kg every 5 weeks (n = 10); group 4 (SSCT-A), mice subjected to a standard SSCT performed 5 weeks after a single injection of busulfan 40 mg/kg (n = 10); group 5 (SSCT-B), mice subjected to a delayed SSCT performed 15 weeks after a single injection of busulfan 40 mg/kg (n = 10); and group 6 (SSCT-C), mice subjected to a delayed SSCT with two additional doses of busulfan 10 mg/kg every 5 weeks (n = 10). Spermatogenic recovery in standard SSCT-A and SSCT-C groups ranged between 22.29 and 22.65%, compared with a lower recovery rate of 11.54% showed in the SSCT-B group. However, donor contribution resulted higher in standard SSCT-A, representing a 69.71% of cross-sections, compared with the rest of conditions ranging from 34.69 to 35.42%. Overall, we concluded that a delay in the SSCT from the gonadotoxic onset decreases the efficiency of donor-derived spermatogenic recovery in mice.

Datos de la publicación

ISSN/ISSNe:: 1071-2690, 1543-706X
Tipo:: Article
Páginas:: 21-29
DOI:: 10.1007/s11626-020-00531-9
Factor de Impacto:: 0,412 SCImago ℠
Cuartil:: Q3 SCImago ℠

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Filiaciones

Medrano JV:: Instituto de Investigación. Unidad de Medicina Reproductiva, Instituto de Investigación Sanitaria La Fe (IIS La Fe), Av. Fernando Abril Martorell, 106. Torre A, Lab. 6.22, 46026, Valencia, Spain.
Acimovic I:: Department of Biology, Faculty of Medicine, Masaryk University, 62500, Brno, Czech Republic
Navarro-Gomezlechon A:: Instituto de Investigación. Unidad de Medicina Reproductiva, Instituto de Investigación Sanitaria La Fe (IIS La Fe), Av. Fernando Abril Martorell, 106. Torre A, Lab. 6.22, 46026, Valencia, Spain
Noguera I:: Animal Facility, Faculty of Pharmacy, Valencia University, 46015, Valencia, Spain
Pellicer A:: Instituto de Investigación. Unidad de Medicina Reproductiva, Instituto de Investigación Sanitaria La Fe (IIS La Fe), Av. Fernando Abril Martorell, 106. Torre A, Lab. 6.22, 46026, Valencia, Spain

Fundación IVI, 46026, Valencia, Spain
Andrés MM:: Hospital Universitario y Politécnico La Fe, 46026, Valencia, Spain
Novella-Maestre E:: Instituto de Investigación. Unidad de Medicina Reproductiva, Instituto de Investigación Sanitaria La Fe (IIS La Fe), Av. Fernando Abril Martorell, 106. Torre A, Lab. 6.22, 46026, Valencia, Spain

Hospital Universitario y Politécnico La Fe, 46026, Valencia, Spain