Gold Nanoparticle-Assisted Virus Formation by Means of the Delivery of an Oncolytic Adenovirus Genome

Data de publicació: 01/06/2020 Data Ahead of Print: 17/06/2020

Autors de IIS La Fe

Luis Sendra Gisbert

Autor
María José Herrero Cervera

Autor
Consuelo Cháfer Pericás

Autor
Elena Aznar Gimeno

Autor
María Dolores Marcos Martínez

Autor
Ramón Martínez Máñez

Autor
Salvador F Aliño Pellicer

Autor

Autors aliens a IIS La Fe

Miguel, A
Navarro-Plaza, MC
de la Higuera, J
Rojas, LA
Alemany, R

Grups d'Investigació

Farmacogenética

Investigador Principal

María José Herrero Cervera
Perinatología

Leader

Máximo Vento Torres
Unidad Mixta de Investigación en Nanomedicina y Sensores

Leader

Ramón Martínez Máñez

Abstract

Oncolytic adenoviruses are a therapeutic alternative to treat cancer based on their ability to replicate selectively in tumor cells. However, their use is limited mainly by the neutralizing antibody (Nab) immune response that prevents repeated dosing. An alternative to facilitate the DNA access to the tumor even in the presence of anti-viral Nabs could be gold nanoparticles able to transfer DNA molecules. However, the ability of these nanoparticles to carry large DNA molecules, such as an oncolytic adenovirus genome, has not been studied. In this work, gold nanoparticles were functionalized with different amounts of polyethylenimine to transfer in a safe and efficient manner a large oncolytic virus genome. Their transfer efficacy and final effect of the oncolytic virus in cancer cells are studied. For each synthesized nanoparticle, (a) DNA loading capacity, (b) complex size, (c) DNA protection ability, (d) transfection efficacy and (e) cytotoxic effect were studied. We observed that small gold nanoparticles (70-80 nm in diameter) protected DNA against nucleases and were able to transfect the ICOVIR-15 oncolytic virus genome encoded in pLR1 plasmid. In the present work, efficient transgene RNA expression, luciferase activity and viral cytopathic effect on cancer cells are reported. These results suggest gold nanoparticles to be an efficient and safe vector for oncolytic adenovirus genome transfer.

Dades de la publicació

ISSN/ISSNe:: 2079-4991, 2079-4991
Tipus:: Article
Pàgines:: -
DOI:: 10.3390/nano10061183
PubMed:: 32560474
Factor d'Impacte:: 0,919 SCImago ℠
Quartil:: Q1 SCImago ℠

Documents

No hi ha documents

Mètriques

Filiacions

Sendra, L:: Univ Valencia, Dept Pharmacol, Gene Therapy Grp, Fac Med, Av Blasco Ibanez 15, Valencia 46010, Spain

Instituto de Investigación. Inst Invest Sanitaria La Fe, Pharmacogenet Unit, Av Fernando Abril Martorell 106, Valencia 46026, Spain
Miguel, A:: Univ Valencia, Dept Pharmacol, Gene Therapy Grp, Fac Med, Av Blasco Ibanez 15, Valencia 46010, Spain
Navarro-Plaza, MC:: Univ Valencia, Dept Pharmacol, Gene Therapy Grp, Fac Med, Av Blasco Ibanez 15, Valencia 46010, Spain

Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarr, Camino Vera S-N, Valencia 46022, Spain
Herrero, MJ:: Univ Valencia, Dept Pharmacol, Gene Therapy Grp, Fac Med, Av Blasco Ibanez 15, Valencia 46010, Spain

Instituto de Investigación. Inst Invest Sanitaria La Fe, Pharmacogenet Unit, Av Fernando Abril Martorell 106, Valencia 46026, Spain
de la Higuera, J:: Univ Valencia, Dept Pharmacol, Gene Therapy Grp, Fac Med, Av Blasco Ibanez 15, Valencia 46010, Spain

Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarr, Camino Vera S-N, Valencia 46022, Spain
Chafer-Pericas, C:: Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarr, Camino Vera S-N, Valencia 46022, Spain
Aznar, E:: Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarr, Camino Vera S-N, Valencia 46022, Spain

CIBER Bioingn Biomat & Nanomed CIBER BBN, Madrid 28029, Spain

Instituto de Investigación. Univ Politecn Valencia, Inst Invest Sanitaria La Fe, Unidad Mixta Invest Nanomed & Sensores, Av Fernando Abril Martorell 106, Valencia 46026, Spain
Marcos, MD:: Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarr, Camino Vera S-N, Valencia 46022, Spain

CIBER Bioingn Biomat & Nanomed CIBER BBN, Madrid 28029, Spain

Instituto de Investigación. Univ Politecn Valencia, Inst Invest Sanitaria La Fe, Unidad Mixta Invest Nanomed & Sensores, Av Fernando Abril Martorell 106, Valencia 46026, Spain
Martinez-Manez, R:: Univ Valencia, Univ Politecn Valencia, Inst Interuniv Invest Reconocimiento Mol & Desarr, Camino Vera S-N, Valencia 46022, Spain

CIBER Bioingn Biomat & Nanomed CIBER BBN, Madrid 28029, Spain

Instituto de Investigación. Univ Politecn Valencia, Inst Invest Sanitaria La Fe, Unidad Mixta Invest Nanomed & Sensores, Av Fernando Abril Martorell 106, Valencia 46026, Spain
Rojas, LA:: Catalan Inst Oncol IDIBELL, Av Gran Via 199-203, Barcelona 08908, Lhospitalet Llo, Spain
Alemany, R:: Catalan Inst Oncol IDIBELL, Av Gran Via 199-203, Barcelona 08908, Lhospitalet Llo, Spain
Alino, SF:: Univ Valencia, Dept Pharmacol, Gene Therapy Grp, Fac Med, Av Blasco Ibanez 15, Valencia 46010, Spain

Instituto de Investigación. Inst Invest Sanitaria La Fe, Pharmacogenet Unit, Av Fernando Abril Martorell 106, Valencia 46026, Spain

Hosp Univ & Politecn La Fe, Clin Pharmacol Unit, Drug Clin Area, Av Fernando Abril Martorell 106, Valencia 46026, Spain