Portal de investigación
Development and application of affordable SNP typing approaches to genotype Mycobacterium tuberculosis complex strains in low and high burden countries
Fecha de publicación:
Autores de IIS La Fe
Participantes ajenos a IIS La Fe
- Cancino-Munoz, I
- Torres-Puente, M
- Mariner-Llicer, C
- Dogba, J
- Akinseye, V
- Adesokan, K
- Kwaghe, A
- Ejeh, F
- Cadmus, S
- Comas, I
Grupos
Abstract
The Mycobacterium tuberculosis complex (MTBC) comprises the species that causes tuberculosis (TB) which affects 10 million people every year. A robust classification of species, lineages, and sub-lineages is important to explore associations with drug resistance, epidemiological patterns or clinical outcomes. We present a rapid and easy-to-follow methodology to classify clinical TB samples into the main MTBC clades. Approaches are based on the identification of lineage and sub-lineage diagnostic SNP using a real-time PCR high resolution melting assay and classic Sanger sequencing from low-concentrated, low quality DNA. Thus, suitable for implementation in middle and low-income countries. Once we validated our molecular procedures, we characterized a total of 491 biological samples from human and cattle hosts, representing countries with different TB burden. Overall, we managed to genotype similar to 95% of all samples despite coming from unpurified and low-concentrated DNA. Our approach also allowed us to detect zoonotic cases in eight human samples from Nigeria. To conclude, the molecular techniques we have developed, are accurate, discriminative and reproducible. Furthermore, it costs less than other classic typing methods, resulting in an affordable alternative method in TB laboratories.
Datos de la publicación
- ISSN/ISSNe:
- 2045-2322, 2045-2322
- Tipo:
- Article
- Páginas:
- 15343-15343
- Factor de Impacto:
- 1,341 SCImago ℠
- Cuartil:
- Q1 SCImago ℠
SCIENTIFIC REPORTS NATURE PUBLISHING GROUP
Citas Recibidas en Web of Science: 6
Documentos
- No hay documentos
Filiaciones
Proyectos asociados
BIOMARCADORES INFLAMATORIOS Y CARDÍACOS COMO PREDICTORES DE EVENTOS CARDIOVASCULARES Y MORTALIDAD TRAS EL ALTA EN LA NEUMONÍA ADQUIRIDA EN LA COMUNIDAD.
Investigador Principal: ROSARIO MENÉNDEZ VILLANUEVA
PI13/00583 . INSTITUTO DE SALUD CARLOS III . 2014
ESTUDIO EPIDEMIOLÓGICO PROSPECTIVO DE VIGILANCIA HOSPITALARIA DE LA ENFERMEDAD NEUMOCÓCICA INVASORA EN ADULTOS MAYORES DE 18 AÑOS EN ESPAÑA.
Investigador Principal: MIGUEL SALAVERT LLETÍ
PFI-PRE-2010-01 . 2012
Cita
Cancino I,Gil A,Torres M,Mariner C,Dogba J,Akinseye V,Adesokan K,Kwaghe A,Ejeh F,Cadmus S,Comas I. Development and application of affordable SNP typing approaches to genotype Mycobacterium tuberculosis complex strains in low and high burden countries. Sci Rep. 2019. 9. (1):p. 15343-15343. IF:3,998. (1).