Portal de investigación
Morphological and molecular characterization of Paragonimus caliensis Little, 1968 (Trematoda: Paragonimidae) from Medellin and Pichinde, Colombia
Autores de IIS La Fe
Participantes ajenos a IIS La Fe
- Lenis, Carolina
- Galiano, Alicia
- Velez, Imelda
- Velez, ID
- Muskus, Carlos
Grupos
Abstract
Paragonimiasis is a subacute to chronic inflammatory granulomatous lung disease caused by the genus Paragonimus In Latin America Paragonimus mexicanus Miyazaki & Ishii, 1968 is the only confirmed species to cause human infections Paragonimus caliensis Little, 1968 is an uncommon species often regarded as a synonym of P. mexicanus Recently, the study of two types of Paragonimus metacercariae from Costa Rica has provided new molecular and morphological evidence that P. caliensis is a separate species from P. mexicanus In the present study, molecular, morphological and phylogenetic tools have been used to characterize two populations of Paragonimus located at west of Medellin, Antioquia and at Pichinde, Valle del Cauca (type locality of P. caliensis), Colombia Adults and metacercariae obtained from Medellin, and metacercariae from Pichinde were analyzed For morphological observations we used light microscopy and scanning electron microscopy (SEM). Morphology of metacercariae and adults matched with the holotype of P. caliensis. The number and arrangement of sensory papillae in the acetabulum region differs from the morphotypes reported for P. caliensis in Costa Rica Two morphotypes in branching patterns of ovary and two morphotypes in branching patterns of testes were identified The main morphological differences between P. caliensis and P. mexicanus corresponded to the size of gonads and their relative positions in the body, and the occasional presence of a cyst wall in P. caliensis metacercariae The molecular and phylogenetic analyses (using nuclear ribosomal ITS2 and partial cytochrome c oxidase subunit 1 COl sequences) confirmed that P. caliensis from the type locality is the same species from Medellin and Costa Rica Furthermore, these analyses also suggest genetic as well as geographical separation of P. caliensis populations between Colombia and Costa Rica Currently, P. mexicanus and P. caliensis are sympatric in the Colombian Pacific bioregion, and specific diagnosis based on their egg size is not possible Therefore, it is necessary to determine the biogeographic distribution ranges of both species and to implement molecular techniques to establish the role of P. caliensis in human paragonimiasis in Colombia.
Datos de la publicación
- ISSN/ISSNe:
- 0001-706X, 1873-6254
- Tipo:
- Article
- Páginas:
- 95-102
- PubMed:
- 29596790
- Factor de Impacto:
- 1,224 SCImago ℠
- Cuartil:
- Q1 SCImago ℠
ACTA TROPICA ELSEVIER
Citas Recibidas en Web of Science: 7
Documentos
- No hay documentos
Filiaciones
Keywords
- Paragonimus caliensis; Morphological characterization; Molecular characterization; Colombia
Cita
Lenis C,Galiano A,Velez I,Velez ID,Muskus C,MARCILLA A. Morphological and molecular characterization of Paragonimus caliensis Little, 1968 (Trematoda: Paragonimidae) from Medellin and Pichinde, Colombia. Acta Trop. 2018. 183. p. 95-102. IF:2,629. (1).