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  3. In situ RT-PCR Optimized for Electron Microscopy Allows Description of Subcellular Morphology of Target mRNA-Expressing Cells in the Brain

In situ RT-PCR Optimized for Electron Microscopy Allows Description of Subcellular Morphology of Target mRNA-Expressing Cells in the Brain

Fecha de publicación:

Autores de IIS La Fe

Participantes ajenos a IIS La Fe

  • Duran-Moreno, M
  • Fuentes-Maestre, J

Grupos

Abstract

In situ RT-PCR detects and amplifies mRNA (cDNA) while obtaining spatial information of gene expression. When the intended use is an ultrastructural analysis of morphology, the procedure may be technically challenging and quality of tissue dramatically altered by proteolytic digestion and extreme astringency and temperature conditions. We describe a low-damaging protocol of in situ RT-PCR combined to conventional electron microscopy that preserves fine morphology, increases sensitivity, and decreases costs and complexity associated to RNA probes.

Datos de la publicación

ISSN/ISSNe:
1662-5102, 1662-5102

Frontiers in Cellular Neuroscience  FRONTIERS MEDIA SA

Tipo:
Article
Páginas:
141-141
PubMed:
28559798
Factor de Impacto:
2,298 SCImago ℠
Cuartil:
Q1 SCImago ℠

Citas Recibidas en Web of Science: 4

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Keywords

  • gene expression; brain; electron microscopy; immunogold; epoxy resin; pre-embedding; biotin-dUTP; TEM

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