Methodological considerations to determine the effect of exercise on brain-derived neurotrophic factor levels

Data de publicació: 01/02/2015

Autors de IIS La Fe

Jose Cortell Ballester

Autor

Autors aliens a IIS La Fe

Pareja-Galeano, H
Alis, R
Sanchis-Gomar, F
Cabo, H
Gomez-Cabrera, MC
Lucia, A
Vina, J

Abstract

Objectives: Physical exercise up-regulates brain-derived neurotrophic factor (BDNF) in the brain and blood. However, there is yet no consensus about the adequate blood processing conditions to standardize its assessment. We aimed to find a reliable blood sample processing method to determine changes in BDNF due to exercise. Design and methods: Twelve healthy university students performed an incremental cycling test to exhaustion. At baseline, immediately after exercise, and 30 and 60 min of recovery, venous blood was drawn and processed under different conditions, i.e. whole blood, serum coagulated for 10 min and 24 h, total plasma, and platelet-free plasma. BDNF concentration was measured by ELISA. Results: Exercise increased BDNF in whole blood and in serum coagulated for 24 h when corrected by hemoconcentration. We did not find effects of exercise on BDNF in serum coagulated for 10 min or in plasma samples. Plasma shows heterogeneous BDNF values in response to exercise that are not prevented when platelets are eliminated while homogeneous BDNF levels were found in whole blood or serum coagulated for 24 hour samples. Conclusions: In exercise studies, BDNF levels should be adjusted by hemoconcentration. Our data highlight the importance of blood sample selection since the differences between each one affect significantly the BDNF factor changes due to exercise. (C) 2014 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

Dades de la publicació

ISSN/ISSNe:: 0009-9120, 1873-2933
Tipus:: Article
Pàgines:: 162-166
DOI:: 10.1016/j.clinbiochem.2014.11.013
PubMed:: 25464018
Factor d'Impacte:: 0,917 SCImago ℠
Quartil:: Q1 SCImago ℠

Documents

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Mètriques

Filiacions

Pareja-Galeano, H:: Univ Valencia, INCLIVA, Sch Med, Dept Physiol, Valencia 46010, Spain
Alis, R:: Catholic Univ Valencia San Vicente Martir, Sch Med, Valencia, Spain

Catholic Univ Valencia San Vicente Martir, Res Inst Dr Vina Giner, Valencia, Spain
Sanchis-Gomar, F:: Univ Valencia, INCLIVA, Sch Med, Dept Physiol, Valencia 46010, Spain
Cabo, H:: Univ Valencia, INCLIVA, Sch Med, Dept Physiol, Valencia 46010, Spain
Cortell-Ballester, J:: Hosp La Fe, E-46009 Valencia, Spain
Gomez-Cabrera, MC:: Univ Valencia, INCLIVA, Sch Med, Dept Physiol, Valencia 46010, Spain
Lucia, A:: European Univ, Madrid, Spain

Hosp 12 Octubre I 12, Res Inst, Madrid, Spain
Vina, J:: Univ Valencia, INCLIVA, Sch Med, Dept Physiol, Valencia 46010, Spain

Keywords

Plasma; Serum; Whole blood; Enzyme-linked immunosorbent assay; Hemoconcentration

Projectes associats

TRATAMIENTO ANTICIPADO CON GANCICLOVIR DE LA INFECCIÓN ACTIVA POR EL CITOMEGALOVIRUS (CMV) EN EL PACIENTE CRÍTICO EN VENTILACIÓN MECÁNICA CON SEPSIS GRAVE O SHOCK SÉPTICO.

Investigador Principal: MIGUEL ANGEL CHIVELI MONLEON

GANCMV-2011