Preterm Gut Microbiome Depending on Feeding Type: Significance of Donor Human Milk

Fecha de publicación: 27/06/2018

Autores de IIS La Fe

Participantes ajenos a IIS La Fe

Alcantara, C
Collado, MC

Grupos

Perinatología

Leader

Máximo Vento Torres

Abstract

Preterm microbial colonization is affected by gestational age, antibiotic treatment, type of birth, but also by type of feeding. Breast milk has been acknowledged as the gold standard for human nutrition. In preterm infants breast milk has been associated with improved growth and cognitive development and a reduced risk of necrotizing enterocolitis and late onset sepsis. In the absence of their mother's own milk (MOM), pasteurized donor human milk (DHM) could be the best available alternative due to its similarity to the former. However, little is known about the effect of DHM upon preterm microbiota and potential biological implications. Our objective was to determine the impact of DHM upon preterm gut microbiota admitted in a referral neonatal intensive care unit (NICU). A prospective observational cohort study in NICU of 69 neonates <32 weeks of gestation and with a birth weight <= 1,500 g was conducted. Neonates were classified in three groups according to feeding practices consisting in their MOM, DHM, or formula. Fecal samples were collected when full enteral feeding (defined as >= 150 cc/kg/day) was achieved. Gut microbiota composition was analyzed by 16S rRNA gene sequencing. Despite the higher variability, no differences in microbial diversity and richness were found, although feeding type significantly influenced the preterm microbiota composition and predictive functional profiles. Preterm infants fed MOM showed a significant greater presence of Bifidobacteriaceae and lower of Staphylococcaceae, Clostridiaceae, and Pasteurellaceae compared to preterm fed DHM. Formula fed microbial profile was different to those observed in preterm fed MOM. Remarkably, preterm infants fed DHM showed closer microbial profiles to preterm fed their MOM. Inferred metagenomic analyses showed higher presence of Bifidobacterium genus in mother's milk group was related to enrichment in the Glycan biosynthesis and metabolism pathway that was not identified in the DHM or in the formula fed groups. In conclusion, DHM favors an intestinal microbiome more similar to MOM than formula despite the differences between MOM and DHM. This may have potential beneficial long-term effects on intestinal functionality, immune system, and metabolic activities.

Datos de la publicación

ISSN/ISSNe:: 1664-302X, 1664-302X
Tipo:: Article
Páginas:: 1376-1376
DOI:: 10.3389/fmicb.2018.01376
PubMed:: 29997594
Factor de Impacto:: 1,633 SCImago ℠
Cuartil:: Q1 SCImago ℠

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Métricas

Filiaciones

Parra-Llorca, A:: Instituto de Investigación. Univ & Polytech Hosp La Fe, Hlth Res Inst La Fe, Neonatal Res Grp, Valencia, Spain
Gormaz, M:: Instituto de Investigación. Univ & Polytech Hosp La Fe, Hlth Res Inst La Fe, Neonatal Res Grp, Valencia, Spain

Univ & Polytech Hosp La Fe, Div Neonatol, Valencia, Spain
Alcantara, C:: Spanish Natl Res Council, Dept Biotechnol, Inst Agrochem & Food Technol, Valencia, Spain
Cernada, M:: Instituto de Investigación. Univ & Polytech Hosp La Fe, Hlth Res Inst La Fe, Neonatal Res Grp, Valencia, Spain

Univ & Polytech Hosp La Fe, Div Neonatol, Valencia, Spain
Nunez-Ramiro, A:: Instituto de Investigación. Univ & Polytech Hosp La Fe, Hlth Res Inst La Fe, Neonatal Res Grp, Valencia, Spain

Univ & Polytech Hosp La Fe, Div Neonatol, Valencia, Spain
Vento, M:: Instituto de Investigación. Univ & Polytech Hosp La Fe, Hlth Res Inst La Fe, Neonatal Res Grp, Valencia, Spain

Univ & Polytech Hosp La Fe, Div Neonatol, Valencia, Spain
Collado, MC:: Spanish Natl Res Council, Dept Biotechnol, Inst Agrochem & Food Technol, Valencia, Spain