Biomarkers of oxidative stress derived damage to proteins and DNA in human breast milk

Fecha de publicación: 03/08/2018

Autores de IIS La Fe

Ángel Sánchez Illana

Autor
Anna Parra Llorca

Autor
María Cernada Badía

Autor
María José Gormaz Moreno

Autor
Máximo Vento Torres

Autor
Julia Kuligowski

Autor

Participantes ajenos a IIS La Fe

Escuder-Vieco, D
Pallas-Alonso, CR

Grupos

Perinatología

Leader

Máximo Vento Torres

Abstract

Oxidative stress derived biomarkers have been extensively studied for assessing pathological situations in the neonatal period and their usefulness for an early outcome prediction of oxidative stress related diseases in non-invasive biofluids could be demonstrated. This is the first report on a method for the simultaneous detection of a panel of oxidative stress related biomarkers for quantifying damage to proteins and DNA in human breast milk samples. A straightforward sample clean-up procedure using 1.5mL of milk was developed and extracts were analyzed by ultra-performance liquid chromatography coupled to tandem mass spectrometry. The analytical method was validated and applied to human milk samples from a cohort of mothers of preterm infants, as well as donor human milk samples before and after pasteurization at a human milk bank. The results demonstrate the feasibility of this method for the analysis of a panel of oxidative stress related biomarkers, reporting ranges found in human milk. (C) 2018 Elsevier B.V. All rights reserved.

Datos de la publicación

ISSN/ISSNe:: 0003-2670, 1873-4324
Tipo:: Article
Páginas:: 78-85
DOI:: 10.1016/j.aca.2018.01.054
Factor de Impacto:: 1,467 SCImago ℠
Cuartil:: Q1 SCImago ℠

Documentos

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Métricas

Filiaciones

Sanchez-Illana, A:: Instituto de Investigación. Hosp La Fe, Hlth Res Inst, Neonatal Res Unit, Avda Fernando Abril Martorell 106, Valencia 46026, Spain
Parra-Llorca, A:: Instituto de Investigación. Hosp La Fe, Hlth Res Inst, Neonatal Res Unit, Avda Fernando Abril Martorell 106, Valencia 46026, Spain
Escuder-Vieco, D:: Hosp 12 Octubre, Serv Neonatol, Banco Reg Leche Humana, Avda Cordoba S-N, Madrid 28041, Spain
Pallas-Alonso, CR:: Hosp 12 Octubre, Serv Neonatol, Banco Reg Leche Humana, Avda Cordoba S-N, Madrid 28041, Spain

Univ Complutense Madrid, Dept Pediat, Fac Med, E-28040 Madrid, Spain
Cernada, M:: Univ & Polytech Hosp La Fe, Div Neonatol, Avda Fernando Abril Martorell 106, Valencia 46026, Spain
Gormaz, M:: Univ & Polytech Hosp La Fe, Div Neonatol, Avda Fernando Abril Martorell 106, Valencia 46026, Spain
Vento, M:: Instituto de Investigación. Hosp La Fe, Hlth Res Inst, Neonatal Res Unit, Avda Fernando Abril Martorell 106, Valencia 46026, Spain

Univ & Polytech Hosp La Fe, Div Neonatol, Avda Fernando Abril Martorell 106, Valencia 46026, Spain
Kuligowski, J:: Instituto de Investigación. Hosp La Fe, Hlth Res Inst, Neonatal Res Unit, Avda Fernando Abril Martorell 106, Valencia 46026, Spain

Keywords

Oxidative stress biomarkers; Liquid chromatography-tandem mass; spectrometry (LC-MS/MS); Human breast milk; Donor human milk; Pre-term newborn; Pasteurization