On-Capillary Surface-Enhanced Raman Spectroscopy: Determination of Glutathione in Whole Blood Microsamples

Data de publicació: 07/08/2018

Autors de IIS La Fe

Ángel Sánchez Illana

Autor
Mayra Pilar Rubio Martinez

Autor
Daniel Cuesta Garcia

Autor
Jose David Piñeiro Ramos

Autor
Máximo Vento Torres

Autor
Guillermo Quintas Soriano

Autor
Julia Kuligowski

Autor

Autors aliens a IIS La Fe

Cantarero, Andres
de la Guardia, Miguel
Lendl, Bernhard

Grups d'Investigació

Perinatología

Leader

Máximo Vento Torres

Abstract

Oxidative stress monitoring in the neonatal period supports early outcome prediction and treatment. Glutathione (GSH) is the most abundant antioxidant in most cells and tissues, including whole blood, and its usefulness as a biomarker has been known for decades. To date, the available methods for GSH determination require laborious sample processing and the use of sophisticated laboratory equipment. To the best of our knowledge, no tools suitable for point-of-care (POC) sensing have been reported. Surface-enhanced Raman spectroscopy (SERS), performed in a microvolume capillary measurement cell, is proposed in this study as a robust approach for the quantification of GSH in human whole blood samples. The use of a silver colloid allowed a highly selective signal enhancement for GSH providing analytical enhancement factors of 3 to 4 orders of magnitude. A highly accurate determination of GSH in whole blood samples with recoveries ranging from 99 to 107% and relative standard deviations less than or equal to 18% were achieved by signal normalization with the intensity of an isotopically labeled internal standard. GSH concentrations were retrieved within 4 min using small-volume blood samples (2 mu L). The developed procedure was applied to the analysis of blood of 20 healthy adults and 36 newborns, obtaining comparable results between literature and those found by SERS and a reference method. The characteristics of this novel tool are suitable for its implementation in a portable optical sensor device enabling POC testing of oxidative stress levels in newborns.

Dades de la publicació

ISSN/ISSNe:: 0003-2700, 1520-6882
Tipus:: Article
Pàgines:: 9093-9100
DOI:: 10.1021/acs.analchem.8b01492
PubMed:: 29939015
Factor d'Impacte:: 2,113 SCImago ℠
Quartil:: Q1 SCImago ℠

Documents

No hi ha documents

Mètriques

Filiacions

Sanchez-Illana, Angel:: Instituto de Investigación. Hlth Res Inst La Fe, Neonatal Res Unit, Avda Fernando Abril Martorell 106, Valencia 46026, Spain
Mayr, Fritz:: Instituto de Investigación. Hlth Res Inst La Fe, Neonatal Res Unit, Avda Fernando Abril Martorell 106, Valencia 46026, Spain
Cuesta-Garcia, Daniel:: Instituto de Investigación. Hlth Res Inst La Fe, Neonatal Res Unit, Avda Fernando Abril Martorell 106, Valencia 46026, Spain

Univ Valencia, Mol Sci Inst, POB 22085, Valencia 46071, Spain
Pineiro-Ramos, Jose David:: Instituto de Investigación. Hlth Res Inst La Fe, Neonatal Res Unit, Avda Fernando Abril Martorell 106, Valencia 46026, Spain
Cantarero, Andres:: Univ Valencia, Mol Sci Inst, POB 22085, Valencia 46071, Spain
de la Guardia, Miguel:: Univ Valencia, Dept Analyt Chem, 50 Dr Moliner St, E-46100 Valencia, Spain
Vento, Maximo:: Instituto de Investigación. Hlth Res Inst La Fe, Neonatal Res Unit, Avda Fernando Abril Martorell 106, Valencia 46026, Spain

Univ & Polytechn Hosp La Fe, Div Neonatol, Avda Fernando Abril Martorell 106, Valencia 46026, Spain
Lendl, Bernhard:: Vienna Univ Technol, Inst Chem Technol & Analyt, Getreidemarkt 9-151, A-1060 Vienna, Austria
Quintas, Guillermo:: Instituto de Investigación. Hlth Res Inst La Fe, Analyt Unit, Avda Fernando Abril Martorell 106, Valencia 46026, Spain

Leitat Technol Ctr, Hlth & Biomed, Avda Fernando Abril Martorell 106, Valencia 46026, Spain
Kuligowski, Julia:: Instituto de Investigación. Hlth Res Inst La Fe, Neonatal Res Unit, Avda Fernando Abril Martorell 106, Valencia 46026, Spain

Keywords

OXIDATIVE STRESS; BIOLOGICAL SAMPLES; AMINO-ACIDS; OXYGEN SUPPLEMENTATION; OXIDIZED GLUTATHIONE; AQUEOUS-SOLUTIONS; PRETERM INFANTS; SENSING METHOD; SCATTERING; SERS