DHA reduces oxidative stress following hypoxia-ischemia in newborn piglets: a study of lipid peroxidation products in urine and plasma

Autores de IIS La Fe

• 

  Consuelo Cháfer Pericás

  Autor

• 

  Máximo Vento Torres

  Autor

Participantes ajenos a IIS La Fe

• Huun, MU
• Garberg, HT
• Escobar, J
• Holme, IM
• Saugstad, OD
• Solberg, R

Grupos

• Perinatología

  

  Leader

  Máximo Vento Torres

Abstract

Background: Lipid peroxidation mediated by reactive oxygen species is a major contributor to oxidative stress. Docosahexaenoic acid (DHA) has anti-oxidant and -neuroprotective properties. Our objective was to assess how oxidative stress measured by lipid peroxidation was modified by DHA in a newborn piglet model of hypoxia-ischemia (HI). Methods: Fifty-five piglets were randomized to (i) hypoxia, (ii) DHA, (iii) hypothermia, (iv) hypothermia + DHA or (v) sham. All groups but sham were subjected to hypoxia by breathing 8% O-2. DHA was administered 210 min after end of hypoxia and the piglets were euthanized 9.5 h after end of hypoxia. Urine and blood were harvested at these two time points and analyzed for F-4-neuroprostanes, F-2-isoprostanes, neurofuranes and isofuranes using UPLC-MS/MS. Results: F-4-neuroprostanes in urine were significantly reduced (P = 0.006) in groups receiving DHA. Hypoxia (median, IQR 1652 nM, 610-4557) vs. DHA (440 nM, 367-738, P = 0.016) and hypothermia (median, IQR 1338 nM, 744-3085) vs. hypothermia + DHA (356 nM, 264-1180, P = 0.006). The isoprostane compound 8-iso-PGF2 alpha was significantly lower (P = 0.011) in the DHA group compared to the hypoxia group. No significant differences were found between the groups in blood. Conclusion: DHA significantly reduces oxidative stress by measures of lipid peroxidation following HI in both normothermic and hypothermic piglets.

Keywords

• Docosahexaenoic acid (DHA); hypoxia-ischemia (HI); isoprostanes; lipid peroxidation; neuroprostanes; neuroprotection; oxidative stress; reactive oxygen species