Fluoroquinolone photodegradation influences specific basophil activation.

Autores de IIS La Fe

• Inmaculada Andreu Ros

  Autor

• Lucia Doñate Bertolin

  Autor

• Francisco Javier Montañez Aguilera

  Autor

• Miguel Ángel Miranda Alonso

  Autor

Participantes ajenos a IIS La Fe

• Mayorga C
• Aranda A
• Blanca-Lopez N
• Ariza A
• Nuin E
• Blanca M
• Torres MJ

Grupos

• Unidad Mixta de Investigación en Mecanismos moleculares de las Reacciones adversas de fármacos

Abstract

Fluoroquinolones (FQs) are photoreactive drugs, but it is not known whether laboratory light exposure can influence the induction of photoproducts and modify in vitro test results. The basophil activation test (BAT) has proven to be useful for evaluating immunoglobulin E (IgE)-mediated hypersensitivity to FQs, with a higher percentage of positive responders with ciprofloxacin (CIP) than with moxifloxacin (MOX). We studied the effect of laboratory light on CIP and MOX degradation, and drug-protein conjugate formation, and its influence on the BAT for evaluating IgE-mediated hypersensitivity to FQs. The results showed an important decrease in fluorescence emission intensity under light compared to dark conditions for MOX, and that BAT positivity was lower in light (17.9%) than in dark (35.7%) conditions. No changes were found for CIP in either fluorescence emission intensity or BAT results (46.4% in both conditions). We can conclude that light exposure is a critical factor in BAT results when photolabile drugs like MOX are used. Therefore, light is important when interpreting in vitro results.