Evolution of Energy Related Metabolites in Plasma from Newborns with Hypoxic-Ischemic Encephalopathy during Hypothermia Treatment

Fecha de publicación: 06/12/2017

Autores de IIS La Fe

Ángel Sánchez Illana

Autor
Antonio Guillermo Núñez Ramiro

Autor
María Cernada Badía

Autor
Anna Parra Llorca

Autor
Eva Maria Valverde Nuñez

Autor
María Teresa Moral Pumarega

Autor
Fernando Cabañas Gonzalez

Autor
Andres Pavon Rodriguez

Autor
Beatriz Perez Benavente

Autor
Ana Alejandra García Robles

Autor
Julia Kuligowski

Autor
Máximo Vento Torres

Autor

Participantes ajenos a IIS La Fe

Blanco, D
Boix, H
Chaffanel, M
Tofe, I
Loureiro, B
Fernandez-Lorenzo, JR
Fernandez-Colomer, B
HYPOTOP Study Group

Grupos

Perinatología

Leader

Máximo Vento Torres

Abstract

Therapeutic hypothermia (TH) initiated within 6 h from birth is the most effective therapeutic approach for moderate to severe hypoxic-ischemic encephalopathy (HIE). However, underlying mechanisms and effects on the human metabolism are not yet fully understood. This work aims at studying the evolution of several energy related key metabolites in newborns with HIE undergoing TH employing gas chromatography - mass spectrometry. The method was validated following stringent FDA requirements and applied to 194 samples from a subgroup of newborns with HIE (N = 61) enrolled in a multicenter clinical trial (HYPOTOP) for the determination of lactate, pyruvate, ketone bodies and several Krebs cycle metabolites at different sampling time points. The analysis of plasma samples from newborns with HIE revealed a decrease of lactate, pyruvate and beta-hydroxybutyrate concentrations, whereas rising malate concentrations were observed. In healthy control newborns (N = 19) significantly lower levels of pyruvate and lactate were found in comparison to age-matched newborns with HIE undergoing TH, whereas acetoacetate and beta-hydroxybutyrate levels were clearly increased. Access to a validated analytical method and a controlled cohort of newborns with HIE undergoing hypothermia treatment for the first time allowed the in-depth study of the evolution of key metabolites of metabolic junctions in this special population.

Datos de la publicación

ISSN/ISSNe:: 2045-2322, 2045-2322
Tipo:: Article
Páginas:: 17039-17039
DOI:: 10.1038/s41598-017-17202-7
Factor de Impacto:: 1,533 SCImago ℠
Cuartil:: Q1 SCImago ℠

Documentos

No hay documentos

Métricas

Filiaciones

Sanchez-Illana, A:: Instituto de Investigación. Neonatal Research Unit, Health Research Institute La Fe, Valencia, Avda. Fernando Abril Martorell 106, 46026, Valencia, Spain

Instituto de Investigación. Hlth Res Inst La Fe, Neonatal Res Unit, Avda Fernando Abril Martorell 106, Valencia 46026, Spain
Núñez-Ramiro A:: Instituto de Investigación. Neonatal Research Unit, Health Research Institute La Fe, Valencia, Avda. Fernando Abril Martorell 106, 46026, Valencia, Spain
Cernada, M:: Instituto de Investigación. Neonatal Research Unit, Health Research Institute La Fe, Valencia, Avda. Fernando Abril Martorell 106, 46026, Valencia, Spain

Instituto de Investigación. Hlth Res Inst La Fe, Neonatal Res Unit, Avda Fernando Abril Martorell 106, Valencia 46026, Spain
Parra-Llorca, A:: Instituto de Investigación. Neonatal Research Unit, Health Research Institute La Fe, Valencia, Avda. Fernando Abril Martorell 106, 46026, Valencia, Spain

Instituto de Investigación. Hlth Res Inst La Fe, Neonatal Res Unit, Avda Fernando Abril Martorell 106, Valencia 46026, Spain
Valverde, E:: Hospital Universitario La Paz, Paseo de la Castellana 261, 28046, Madrid, Spain

Hosp Univ La Paz, Paseo Castellana 261, Madrid 28046, Spain
Blanco, D:: Hospital Universitario Gregorio Marañón, C/O'Donnell 48-50, 28009, Madrid, Spain

Hosp Univ Gregorio Maranon, C-O Donnell 48-50, Madrid 28009, Spain
Moral-Pumarega, MT:: Hospital Universitario 12 de Octubre, Avda. de Córdoba, s/n, 28041, Madrid, Spain

Hosp Univ 12 Octubre, Avda Cordoba S-N, Madrid 28041, Spain
Cabañas F:: Hospital Universitario Quirónsalud Madrid, Calle Diego de Velazquez s/n, 28223, Madrid, Spain
Boix, H:: Hospital Universitario Vall d'Hebron, Passeig Vall d'Hebron 119-129, 08035, Barcelona, Spain

Hosp Univ Vall dHebron, Passeig Vall dHebron 119-129, Barcelona 08035, Spain
Pavon, A:: Hospital Universitario Virgen del Rocío, Avda. Manuel Siurot, s/n, 41013, Sevilla, Spain

Hosp Univ Virgen del Rocio, Avda Manuel Siurot S-N, Seville 41013, Spain
Chaffanel, M:: Hospital Materno Infantil Carlos Haya, Avda. Arroyo de los Angeles s/n, 29011, Málaga, Spain

Hosp Materno Infantil Carlos Haya, Avda Arroyo Angeles S-N, Malaga 29011, Spain
Benavente-Fernandez, I:: Hospital Universitario Puerta del Mar, Avda. Ana de Viya 21, 11009, Cádiz, Spain

Hosp Univ Puerta Mar, Avda Ana Viya 21, Cadiz 11009, Spain
Tofe, I:: Hospital Universitario Reina Sofía, Avda. Menendez Pidal s/n, 14004, Córdoba, Spain

Hosp Univ Reina Sofia, Avda Menendez Pidal S-N, Cordoba 14004, Spain
Loureiro, B:: Hospital Universitario Cruces, Plaza Cruces s/n, 48903, Barakaldo, Vizcaya, Spain

Hosp Univ Cruces, Plaza Cruces S-N, Baracaldo 48903, Vizcaya, Spain
Fernandez-Lorenzo, JR:: Hospital Álvaro Cunqueiro, Estrada Clara Campoamor 341, 36312, Vigo, Spain

Hosp Alvaro Cunqueiro, Estr Clara Campoamor 341, Vigo 36312, Spain
Fernandez-Colomer, B:: Hospital Central de Asturias, Avda. De Roma, 33011, Oviedo, Asturias, Spain

Hosp Cent Asturias, Avda De Roma, Asturias 33011, Spain
Garcia-Robles, A:: Instituto de Investigación. Neonatal Research Unit, Health Research Institute La Fe, Valencia, Avda. Fernando Abril Martorell 106, 46026, Valencia, Spain

Instituto de Investigación. Hlth Res Inst La Fe, Neonatal Res Unit, Avda Fernando Abril Martorell 106, Valencia 46026, Spain
Kuligowski, J:: Instituto de Investigación. Neonatal Research Unit, Health Research Institute La Fe, Valencia, Avda. Fernando Abril Martorell 106, 46026, Valencia, Spain.

Instituto de Investigación. Hlth Res Inst La Fe, Neonatal Res Unit, Avda Fernando Abril Martorell 106, Valencia 46026, Spain
Vento, M:: Division of Neonatology, University & Polytechnic Hospital La Fe, Avda. Fernando Abril Martorell 106, 46026, Valencia, Spain.

Instituto de Investigación. Hlth Res Inst La Fe, Neonatal Res Unit, Avda Fernando Abril Martorell 106, Valencia 46026, Spain

Univ & Polytech Hosp La Fe, Div Neonatol, Avda Fernando Abril Martorell 106, Valencia 46026, Spain
HYPOTOP Study Group:: HYPOTOP Study Group

Keywords

CHROMATOGRAPHY-MASS SPECTROMETRY; TRICARBOXYLIC-ACID CYCLE; THERAPEUTIC HYPOTHERMIA; CARBOXYLIC-ACIDS; BRAIN-DAMAGE; LACTATE; GLYCOLYSIS; STABILITY; CAPILLARY; ASPHYXIA