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Intracoronary Administration of Allogeneic Adipose Tissue-Derived Mesenchymal Stem Cells Improves Myocardial Perfusion But Not Left Ventricle Function, in a Translational Model of Acute Myocardial Infarction
Data de publicació:
Autors de IIS La Fe
Autors aliens a IIS La Fe
- Bobi, J
- Solanes, N
- Fernandez-Jimenez, R
- Galan-Arriola, C
- Dantas, AP
- Fernandez-Friera, L
- Galvez-Monton, C
- Rigol-Monzo, E
- Ramirez, J
- Roque, M
- Bayes-Genis, A
- Sanchez-Gonzalez, J
- Garcia-Alvarez, A
- Sabate, M
- Roura, S
- Ibáñez B
- Rigol, M
Grups d'Investigació
Abstract
Background-Autologous adipose tissue-derived mesenchymal stem cells (ATMSCs) therapy is a promising strategy to improve post-myocardial infarction outcomes. In a porcine model of acute myocardial infarction, we studied the long-term effects and the mechanisms involved in allogeneic ATMSCs administration on myocardial performance. Methods and Results-Thirty-eight pigs underwent 50 minutes of coronary occlusion; the study was completed in 33 pigs. After reperfusion, allogeneic ATMSCs or culture medium (vehicle) were intracoronarily administered. Follow-ups were performed at short (2 days after acute myocardial infarction vehicle-treated, n=10; ATMSCs-treated, n=9) or long term (60 days after acute myocardial infarction vehicle-treated, n=7; ATMSCs-treated, n=7). At short term, infarcted myocardium analysis showed reduced apoptosis in the ATMSCs-treated animals (48.6 +/- 6% versus 55.9 +/- 5.7% in vehicle; P=0.017); enhancement of the reparative process with up-regulated vascular endothelial growth factor, granulocyte macrophage colony-stimulating factor, and stromal-derived factor-1 alpha gene expression; and increased M2 macrophages (67.2 +/- 10% versus 54.7 +/- 10.2% in vehicle; P=0.016). In long-term groups, increase in myocardial perfusion at the anterior infarct border was observed both on day-7 and day-60 cardiac magnetic resonance studies in ATMSCs-treated animals, compared to vehicle (87.9 +/- 28.7 versus 57.4 +/- 17.7 mL/min per gram at 7 days; P=0.034 and 99 +/- 22.6 versus 43.3 +/- 14.7 22.6 mL/min per gram at 60 days; P=0.0001, respectively). At day 60, higher vascular density was detected at the border zone in the ATMSCs-treated animals (118 +/- 18 versus 92.4 +/- 24.3 vessels/mm(2) in vehicle; P=0.045). Cardiac magnetic resonance-measured left ventricular ejection fraction of left ventricular volumes was not different between groups at any time point. Conclusions-In this porcine acute myocardial infarction model, allogeneic ATMSCs-based therapy was associated with increased cardioprotective and reparative mechanisms and with better cardiac magnetic resonance-measured perfusion. No effect on left ventricular volumes or ejection fraction was observed.
Dades de la publicació
- ISSN/ISSNe:
- 2047-9980, 2047-9980
- Tipus:
- Article
- Pàgines:
- -
- PubMed:
- 28468789
- Factor d'Impacte:
- 2,674 SCImago ℠
- Quartil:
- Q1 SCImago ℠
Journal of the American Heart Association WILEY
Cites Rebudes en Web of Science: 40
Documents
- No hi ha documents
Filiacions
Keywords
- adipose tissue-derived mesenchymal stem cells; allogeneic origin; myocardial infarction; myocardial perfusion; vascular density
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