Human Mesenchymal Stem Cells Growth and Osteogenic Differentiation on Piezoelectric Poly(vinylidene fluoride) Microsphere Substrates

Fecha de publicación: 11/11/2017

Autores de IIS La Fe

Lourdes Cordón Gallego

Autor
Sergio Doria Belenguer

Autor
María Leonor Senent Peris

Autor
Amparo Sempere Talens

Autor

Participantes ajenos a IIS La Fe

Sobreiro-Almeida, R.
Tamaño-Machiavello MN
Carvalho, E. O.
Correia, D. M.
Ribeiro, C.
Lanceros-Mendez, S.
Sabater i Serra, R.
Gomez Ribelles, J. L.

Grupos

Hematología y Hemoterapia

Investigador Principal

Javier De La Rubia Comos

Abstract

The aim of this work was to determine the influence of the biomaterial environment on human mesenchymal stem cell (hMSC) fate when cultured in supports with varying topography. Poly(vinylidene fluoride) (PVDF) culture supports were prepared with structures ranging between 2D and 3D, based on PVDF films on which PVDF microspheres were deposited with varying surface density. Maintenance of multipotentiality when cultured in expansion medium was studied by flow cytometry monitoring the expression of characteristic hMSCs markers, and revealed that cells were losing their characteristic surface markers on these supports. Cell morphology was assessed by scanning electron microscopy (SEM). Alkaline phosphatase activity was also assessed after seven days of culture on expansion medium. On the other hand, osteoblastic differentiation was monitored while culturing in osteogenic medium after cells reached confluence. Osteocalcin immunocytochemistry and alizarin red assays were performed. We show that flow cytometry is a suitable technique for the study of the differentiation of hMSC seeded onto biomaterials, giving a quantitative reliable analysis of hMSC-associated markers. We also show that electrosprayed piezoelectric poly(vinylidene fluoride) is a suitable support for tissue engineering purposes, as hMSCs can proliferate, be viable and undergo osteogenic differentiation when chemically stimulated.

Datos de la publicación

ISSN/ISSNe:: 1661-6596, 1661-6596
Tipo:: Article
Páginas:: -
DOI:: 10.3390/ijms18112391
Factor de Impacto:: 1,260 SCImago ℠
Cuartil:: Q1 SCImago ℠

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Filiaciones

Sobreiro-Almeida, R.:: Centro/Departamento de Física, Universidade do Minho, 4710-057 Braga, Portugal.

Univ Minho, Ctr Dept Fis, P-4710057 Braga, Portugal
Tamaño-Machiavello MN:: Centre for Biomaterials and Tissue Engineering, CBIT, Universitat Politècnica de València, 46022 Valencia, Spain.
Carvalho, E. O.:: Univ Minho, Ctr Dept Fis, P-4710057 Braga, Portugal
Cordon, L.:: Instituto de Investigación. Hematology Research Group, Instituto de Investigación Sanitaria La Fe, 46026 Valencia, Spain.

Centro de Investigación Biomédica en Red de Cáncer (CIBERONC), Instituto Carlos III, 28029 Madrid, Spain.

Instituto de Investigación. Inst Invest Sanitaria La Fe, Hematol Res Grp, Valencia 46026, Spain

Inst Carlos III, Ctr Invest Biomed Red Canc CIBERONC, Madrid 28029, Spain
Doria, S.:: Univ Politecn Valencia, CBIT, Ctr Biomat & Tissue Engn, E-46022 Valencia, Spain
Senent, L.:: Hematology Department, Hospital Universitario y Politécnico La Fe, 46026 Valencia, Spain.

Instituto de Investigación. Inst Invest Sanitaria La Fe, Hematol Res Grp, Valencia 46026, Spain

Inst Carlos III, Ctr Invest Biomed Red Canc CIBERONC, Madrid 28029, Spain

Hosp Univ & Politecn La Fe, Dept Hematol, Valencia 46026, Spain
Correia, D. M.:: Centro/Departamento de Química, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal.

Univ Minho, Ctr Dept Fis, P-4710057 Braga, Portugal

Univ Minho, Ctr Dept Quim, Campus Gualtar, P-4710057 Braga, Portugal
Ribeiro, C.:: CEB-Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.

Univ Minho, Ctr Dept Fis, P-4710057 Braga, Portugal

Univ Minho, CEB Ctr Biol Engn, Campus Gualtar, P-4710057 Braga, Portugal
Lanceros-Mendez, S.:: BCMaterials, Parque Científico y Tecnológico de Bizkaia, 48160 Derio, Spain.

IKERBASQUE, Basque Foundation for Science, 48013 Bilbao, Spain.

BCMaterials, Parque Cient & Tecnol Bizkaia, Derio 48160, Spain

Basque Fdn Sci, IKERBASQUE, Bilbao 48013, Spain
Sabater i Serra, R.:: Biomedical Research Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 46022 Valencia, Spain.
Sempere, A.:: Instituto de Investigación. Inst Invest Sanitaria La Fe, Hematol Res Grp, Valencia 46026, Spain

Inst Carlos III, Ctr Invest Biomed Red Canc CIBERONC, Madrid 28029, Spain

Hosp Univ & Politecn La Fe, Dept Hematol, Valencia 46026, Spain